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PMID: 6780757 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mistranslation in cells infected with the bacteriophage MS2: direct evidence of Lys for Asn substitution.

Molecular & general genetics : MGG ·Vol. 180 ·No. 2 ·1980-00-00 ·Pages 275-81

Parker J, Johnston TC, Borgia PT

Abstract

The coat protein of the bacteriophage MS2 was found to show an increased level of charge heterogeneity when synthesized in Escherichia coli starved for Asn or Lys. No such increase was found when the host was starved for Arg, His Ile or Pro. This is the pattern predicted by "two-out-of-three" codon misreading in the coat protein gene. In the case of Asn starvation, direct measurements of the relative incorporation of Lys demonstrate that the observed charge heterogeneity is the result of mistranslation. Asn starvation increased the error frequency in coat protein to over 0.3 mistake per asparagine codon. The small amount of charge heterogeneity seen in unstarved cells seems also to be the result of misreading Asn codons.

MeSH Terms
Asparagine/genetics,metabolism Capsid/biosynthesis Codon Coliphages/genetics Escherichia coli/genetics,metabolism Genetic Code Lysine/genetics,metabolism Protein Biosynthesis RNA Phages/genetics RNA, Viral/metabolism Viral Proteins/biosynthesis
Chemicals
Codon RNA, Viral Viral Proteins Asparagine Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Parker J
Johnston T C
Borgia P T
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425839
Published
1980-00-00
Pages
275-81
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM25855 · United States
External Links
PubMed source DOI: 10.1007/BF00425839
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