The contribution of hydrogen peroxide resistance to virulence of Mycobacterium tuberculosis during the first six days after intravenous infection of normal and BCG-vaccinated guinea-pigs.

British journal of experimental pathology ·Vol. 62 ·No. 1 ·1981-02-00 ·Pages 34-40

Jackett PS, Aber VR, Mitchison DA, Lowrie DB

Abstract

The course of infection with Mycobacterium tuberculosis strains H37Rv, H37Ra and their isoniazid-resistant, hydrogen peroxide-susceptible mutants in guinea-pig spleen and lung were assessed by measuring changes in number of viable bacteria during the first and second 3-day intervals after i.v. infection of normal and BCG-vaccinated animals. Vaccination had no effect on bacterial survival in the first 3 days of infection. The peroxide-susceptible mutants were killed or inhibited more than their parent strains; in normal animals this enhanced susceptibility was expressed equally during the first and second 3-day intervals while in vaccinated animals the effect was greater in the second 3-day interval. The results suggest that hydrogen peroxide is generated in significant amounts in the environment of tubercle bacilli lodged in normal tissues and in enhanced amounts when acquired immunity becomes expressed after a few days' lodgement in the tissues of vaccinated animals. Thus hydrogen peroxide resistance may contribute to virulence by protecting against both normal resident and immunologically activated macrophages.

MeSH Terms

Animals BCG Vaccine Drug Resistance, Microbial Female Guinea Pigs Hydrogen Peroxide/pharmacology Isoniazid/pharmacology Lung/microbiology Mutation Mycobacterium tuberculosis/drug effects,genetics Spleen/microbiology Time Factors Tuberculosis/microbiology,prevention & control Virulence

Chemicals

BCG Vaccine Hydrogen Peroxide Isoniazid

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jackett P S

Aber V R

Mitchison D A

Lowrie D B

References (20)

20 references, click to expand

Effect of hyperoxia on superoxide anion and hydrogen peroxide production of polymorphonuclear leucocytes and alveolar macrophages.
Br J Haematol. 1977 Jun;36(2):241-8 PMID: 194622
Hydrogen peroxide release by rat alveolar macrophages: comparison with blood neutrophils.
Infect Immun. 1978 Feb;19(2):621-9 PMID: 564878
Increased superoxide anion production by immunologically activated and chemically elicited macrophages.
J Exp Med. 1978 Jul 1;148(1):115-27 PMID: 209122
VIRULENCE IN THE GUINEA-PIG, SUSCEPTIBILITY TO HYDROGEN PEROXIDE, AND CATALASE ACTIVITY OF ISONIAZID-SENSITIVE TUBERCLE BACILLI FROM SOUTH INDIAN AND BRITISH PATIENTS.
J Pathol Bacteriol. 1963 Oct;86:377-86 PMID: 14068947
Cellular hypersensitivity and cellular immunity in the pathogensis of tuberculosis: specificity, systemic and local nature, and associated macrophage enzymes.
Bacteriol Rev. 1968 Jun;32(2):85-102 PMID: 4873814
New substrates for the fluorometric determination of oxidative enzymes.
Anal Chem. 1968 Jul;40(8):1256-63 PMID: 5743279
Virulence and resistance to superoxide, low pH and hydrogen peroxide among strains of Mycobacterium tuberculosis.
J Gen Microbiol. 1978 Jan;104(1):37-45 PMID: 24084
The virulence in the guinea-pig of tubercle bacilli isolated before treatment from South Indian patients with pulmonary tuberculosis. I. Homogeneity of the investigation and a critique of the virulence test.
Bull World Health Organ. 1961;25:285-312 PMID: 14474648
The relationship between listericidal and mycobacteriostatic activity in BCG-vaccinated mice.
J Reticuloendothel Soc. 1974 Sep;16(3):139-49 PMID: 4218267
Mechanisms of acquired resistance in mouse typhoid.
J Exp Med. 1966 Oct 1;124(4):585-600 PMID: 4958757
The effects of some sulfhydryl compounds on growth of catalase-positive and catalase-negative tubercle bacilli.
Am Rev Tuberc. 1956 Jul;74(1):42-9 PMID: 13327213
The susceptibility of strains of Mycobacterium tuberculosis to catalase-mediated peroxidative killing.
J Gen Microbiol. 1980 Dec;121(2):381-6 PMID: 6790665
Host-parasite relationships in experimental airborne tuberculosis. VII. Fate of Mycobacterium tuberculosis in primary lung lesions and in primary lesion-free lung tissue infected as a result of bacillemia.
J Infect Dis. 1978 Aug;138(2):237-41 PMID: 98601
Mycobacterium tuberculosis strain H37Rv.
J Med Lab Technol. 1969 Oct;26(4):389-90 PMID: 4982234
Influence of infection with Toxoplasma on macrophage function, and role of macrophages in resistance to Toxoplasma.
Am J Trop Med Hyg. 1977 Nov;26(6 Pt 2):170-86 PMID: 339761
Macrophage oxygen-dependent antimicrobial activity. II. The role of oxygen intermediates.
J Exp Med. 1979 Oct 1;150(4):950-64 PMID: 512587
The 7H11 medium for the cultivation of mycobacteria.
Am Rev Respir Dis. 1968 Aug;98(2):295-6 PMID: 4299186
Oxidative metabolic responses of rabbit pulmonary alveolar macrophages.
Blood. 1979 Mar;53(3):486-91 PMID: 760863
Effect of phagocytosis on superoxide anion production and superoxide dismutase levels in BCG-activated and normal rabbit alveolar macrophages.
J Reticuloendothel Soc. 1978 Aug;24(2):101-6 PMID: 212559
Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi.
J Exp Med. 1979 May 1;149(5):1056-68 PMID: 376774

Article Info

Journal

British journal of experimental pathology

Abbr.

Br J Exp Pathol

ISSN

0007-1021

Published

1981-02-00

Pages

34-40

Language

English

Region

England

NLM ID

0372543

PMCID

PMC2041641

Subset

External Links

PubMed source PMC Full Text View journal details

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The contribution of hydrogen peroxide resistance to virulence of Mycobacterium tuberculosis during the first six days after intravenous infection of normal and BCG-vaccinated guinea-pigs.

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