Home LiteratureArticle Details
PMID: 6785044 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutants partially defective in excision repair at five autosomal loci in Drosophila melanogaster.

Chromosoma ·Vol. 82 ·No. 2 ·1981-00-00 ·Pages 249-57

Boyd JB, Harris PV

Abstract

Primary cell cultures derived from mutants in seventeen different genes were analyzed for their ability to excise pyrimidine dimers from DNA. Five of these mutagen-sensitive mutants [mus(2)205A1, mus(3)302D1, mus(3)304D3, mus(3)306D1, mus(3)308D2] display a significantly reduced excision capacity relative to control cultured. In addition, two of the five [mus(3)306D2, mus(3)308D2] are defective in the accumulation of single-strand breaks normally seen after ultraviolet irradiation. This study, therefore, brings the total number of Drosophila mutants known to be defective in excision repair to seven. The results are discussed relative to other genetic and biochemical properties of these mutants.

MeSH Terms
Animals Cells, Cultured DNA Repair Drosophila melanogaster/genetics Genes Mutation Pyrimidine Dimers/metabolism
Chemicals
Pyrimidine Dimers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boyd J B
Harris P V
References (16)
16 references, click to expand
  1. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.
    Proc Natl Acad Sci U S A. 1964 Feb;51:226-31 PMID: 14124320
  2. Excision repair in Drosophila. Analysis of strand breaks appearing in DNA of mei-9 mutants following mutagen treatment.
    Biochim Biophys Acta. 1980 Nov 14;610(1):116-29 PMID: 6776988
  3. Fractionation of DNA from mammalian cells by alkaline elution.
    Biochemistry. 1976 Oct 19;15(21):4629-37 PMID: 9979
  4. Isolation and identification of the irradiation product of thymine.
    Biochim Biophys Acta. 1960 Jul 15;41:550-1 PMID: 13800233
  5. Genetic controls of meiotic recombination and somatic DNA metabolism in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4140-4 PMID: 825857
  6. The mei-9 alpha mutant of Drosophila melanogaster increases mutagen sensitivity and decreases excision repair.
    Genetics. 1976 Nov;84(3):527-44 PMID: 826452
  7. Defective repair replication of DNA in xeroderma pigmentosum.
    Nature. 1968 May 18;218(5142):652-6 PMID: 5655953
  8. DNA repair in Potorous tridactylus.
    Biophys J. 1974 Oct;14(10):791-803 PMID: 4473053
  9. Removal of pyrimidine dimers from Saccharomyces cerevisiae nuclear DNA under nongrowth conditions as detected by a sensitive, enzymatic assay.
    Mutat Res. 1978 Apr;50(1):43-56 PMID: 347280
  10. A seventh complementation group in excision-deficient xeroderma pigmentosum.
    Mutat Res. 1979 Aug;62(1):183-90 PMID: 492197
  11. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA.
    J Mol Biol. 1964 Aug;9:395-410 PMID: 14202275
  12. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.
    Proc Natl Acad Sci U S A. 1964 Feb;51:293-300 PMID: 14124327
  13. Characterization of postreplication repair in mutagen-sensitive strains of Drosophila melanogaster.
    Genetics. 1976 Nov;84(3):507-26 PMID: 826451
  14. DNA repair in bacteria and mammalian cells.
    Annu Rev Biochem. 1979;48:783-836 PMID: 382997
  15. Repair of ultraviolet light damage in a variety of human fibroblast cell strains.
    Cancer Res. 1977 Mar;37(3):904-10 PMID: 837385
  16. Inhibition of DNA excision repair in human cells by arabinofuranosyl cytosine: effect on normal and xeroderma pigmentosum cells.
    Mol Pharmacol. 1979 Mar;15(2):367-74 PMID: 470933
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00286109
Published
1981-00-00
Pages
249-57
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM22221 · United States
External Links
PubMed source DOI: 10.1007/BF00286109 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2