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PMID: 6787602 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Laminin and fibronectin in cell adhesion: enhanced adhesion of cells from regenerating liver to laminin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2403-6

Carlsson R, Engvall E, Freeman A, Ruoslahti E

Abstract

Laminin, a basement membrane glycoprotein isolated from cultures of mouse endodermal cells and rat yolk sac carcinoma cells, promoted the attachment of liver cells obtained from regenerating mouse liver. Cells from normal mouse liver attached readily to dishes coated with fibronectin but attached poorly to surfaces coated with laminin. Both proteins efficiently promoted the attachment of cells from livers undergoing regeneration. After regeneration, the attachment to laminin returned to the low levels found in animals not subjected to partial hepatectomy but attachment to fibronectin remained high. Immunofluorescent staining of sections of normal liver with antilaminin revealed the presence of laminin in or adjacent to the walls of the bile ducts and blood vessels. After induction of regeneration by partial hepatectomy, increased amounts of laminin appeared in the sinusoidal areas. After carbon tetrachloride poisoning, staining for laminin was especially pronounced in the necrotic and postnecrotic areas around the central veins. This additional expression of laminin was transient. It reached a maximum around 5--6 days after the injury and then gradually disappeared. These findings show that laminin is an adhesive protein. The increase of laminin in regenerating liver and the adhesiveness of cells from such livers to laminin suggest a role for laminin in the maintenance of a proper tissue organization during liver regeneration.

MeSH Terms
Animals Basement Membrane/metabolism Cell Adhesion/drug effects Egtazic Acid/pharmacology Fibronectins/physiology Glycoproteins/physiology Laminin Liver/cytology Liver Regeneration Membrane Proteins/physiology Mice
Chemicals
Fibronectins Glycoproteins Laminin Membrane Proteins Egtazic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carlsson R
Engvall E
Freeman A
Ruoslahti E
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2403
Published
1981-04-00
Pages
2403-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319354
Subset
IM
Grants
NIADDK NIH HHS · AM 26693 · United States
NCI NIH HHS · CA 25392 · United States
NCI NIH HHS · CA 27455 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.4.2403 PMC Full Text View journal details
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