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PMID: 6787608 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of proliferation and differentiation of neoplastic B cells by anti-immunoglobulin and T-cell factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2507-11

Isakson PC, Puré E, Uhr JW, Vitetta ES

Abstract

Sepharose-bound anti-immunoglobulins, which are potent mitogens for normal adult B cells, are not mitogenic for tumor cells freshly isolated from mice carrying the B-cell leukemia BCL1. However, after 4 or more days of in vitro cultivation, BCL1 cells can be stimulated to divide by either anti-mu or anti-delta antibodies. These results suggest that in vitro cultivation of BCL1 cells results in their differentiation into more mature cells which can be triggered to proliferate by their interaction with anti-Ig antibodies. Addition of T-cell helper factors to anti-Ia treated BCL1 cells results in their differentiation into Ig-secreting cells. These results indicate that surface Ig molecules on BCL1 cells are capable of delivering an activation signal to the cells but that the cells require a second signal from T cells for induction of Ig secretion.

MeSH Terms
Animals Antibodies, Anti-Idiotypic Antibody Formation B-Lymphocytes/immunology Cell Differentiation/drug effects Immunoglobulin Idiotypes Interleukin-1 Kinetics Leukemia, Experimental/immunology Lymphocyte Activation Mice Proteins/pharmacology
Chemicals
Antibodies, Anti-Idiotypic Immunoglobulin Idiotypes Interleukin-1 Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Isakson P C
Puré E
Uhr J W
Vitetta E S
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2507
Published
1981-04-00
Pages
2507-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319376
Subset
IM
Grants
NIAID NIH HHS · AI-12789 · United States
NCI NIH HHS · CA-09082 · United States
NCI NIH HHS · CA-23115 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.4.2507 PMC Full Text View journal details
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