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PMID: 6790247 Published · ppublish English Journal Article

Fine cytogenetical analysis of the band 10A1-2 and the adjoining regions in the Drosophila melanogaster X chromosome. I. Cytology of the region and mapping of chromosome rearrangement.

Chromosoma ·Vol. 82 ·No. 1 ·1981-00-00 ·Pages 9-23

Zhimulev IF, Semeshin VF, Belyaeva ES

Abstract

The region 9E1-2 - 10B1-2 of the Drosophila melanogaster X chromosome was analysed under the light (LM) and the electron (EM) microscope using different fixatives and an EM map of the region was constructed. EM analysis revealed 21 bands in the region 9E1-2 - 10B1-2 instead of 36 bands in Bridges' map. This discrepancy mainly results from the fact that 14 bands indicated as "doublets" by Bridges appear as a single bands. No doublets were found in the whole 9B1-2 - 10C1-2 region after fixation of salivary glands in 3% glutaraldehyde, 3% formaldehyde and 3 : 1 ethanol-acetic acid mixture. 45% acetic acid is the only fixative which results in strongly vacuolated appearance of the bands. - The break points of 30 chromosome rearrangements in the region 9E1-2 - 10B1-2 were located under EM or LM within the limits of the EM map of this region.

MeSH Terms
Animals Chromosome Mapping Drosophila melanogaster/genetics Female Microscopy, Electron Sex Chromosomes/ultrastructure X Chromosome/ultrastructure
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhimulev I F
Semeshin V F
Belyaeva E S
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23 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00285746
Published
1981-00-00
Pages
9-23
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00285746 View journal details
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