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PMID: 6790718 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Rapid cataloging of ribonuclease T1 resistant oligonucleotides from ribosomal RNAs for phylogenetic studies.

Journal of molecular evolution ·Vol. 17 ·No. 4 ·1981-00-00 ·Pages 227-36

Stackebrandt E, Ludwig W, Schleifer KH, Gross HJ

Abstract

A rapid and simple method of oligonucleotide cataloging for phylogenetic studies is presented. It involves in vitro 5'-32P-labelling of RNase T1 - resistant oligonucleotides of ribosomal 16S RNA and finger-printing by high voltage electrophoresis and gradient thinlayer chromatography. Oligonucleotide sequences are established by the mobility shift method, using controlled alkali cleavage, high voltage electrophoresis and homochromatography. These procedures facilitate in particular the analysis of long RNase T1 - resistant oligonucleotides. Oligonucleotide catalogs are established fo three Actinomycetes, namely Oerskovia turbata, Actinoplanes philippinensis and Ampullariella regularis. These catalogs are equivalent to those obtained by methods which were described by Sanger and Woese.

MeSH Terms
Actinomyces/genetics Bacteria/genetics Base Sequence Oligonucleotides Oligoribonucleotides Phylogeny RNA, Ribosomal/genetics Ribonuclease T1 Ribonucleases Species Specificity
Chemicals
Oligonucleotides Oligoribonucleotides RNA, Ribosomal Ribonucleases Ribonuclease T1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stackebrandt E
Ludwig W
Schleifer K H
Gross H J
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28 references, click to expand
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF01732760
Published
1981-00-00
Pages
227-36
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01732760 View journal details
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