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PMID: 6793035 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of a myeloma--spleen-cell hybrid producing antibody to phenylalanine hydroxylase.

The Biochemical journal ·Vol. 191 ·No. 3 ·1980-12-01 ·Pages 777-83

Cotton RG, Jennings IG, Choo KH, Fowler K

Abstract

Application of the technique of myeloma--spleen-cell fusion [Kohler & Milstein (1975) Nature (London) 256, 495--497] has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two-dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.

MeSH Terms

Animals Antibody Formation Antibody-Producing Cells/immunology Clone Cells/immunology Cross Reactions Haplorhini Humans Hybrid Cells/immunology Immunodiffusion Immunoelectrophoresis, Two-Dimensional Karyotyping Liver/enzymology Mice Multiple Myeloma/immunology Phenylalanine Hydroxylase/immunology Rats Spleen/immunology

Chemicals

Phenylalanine Hydroxylase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Cotton R G

Jennings I G

Choo K H

Fowler K

References (10)

10 references, click to expand

Letter: Fusion of two immunoglobulin-producing myeloma cells.
Nature. 1973 Jul 6;244(5410):42-3 PMID: 4582485
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
Continuous cultures of fused cells secreting antibody of predefined specificity.
Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
Phenylalanine hydroxylase of Macaca irus A simple purification by affinity chromatography.
Eur J Biochem. 1975 Dec 15;60(2):427-30 PMID: 812698
Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines.
Eur J Immunol. 1976 Apr;6(4):292-5 PMID: 825374
Antibodies to major histocompatibility antigens produced by hybrid cell lines.
Nature. 1977 Apr 7;266(5602):550-2 PMID: 558524
Genetics of the mammalian phenylalanine hydroxylase system. Studies of human liver phenylalanine hydroxylase subunit structure and of mutations in phenylketonuria.
Biochem J. 1979 Aug 1;181(2):285-94 PMID: 496890
Genetics of the mammalian phenylalanine hydroxylase system. II. Immunological and two-dimensional gel electrophoretic studies of phenylalanine hydroxylase in cultured normal and mutant rat hepatoma cells.
Biochem Genet. 1979 Oct;17(9-10):921-46 PMID: 540024
Observations indicating the nature of the mutation in phenylketonuria.
J Inherit Metab Dis. 1980;2(4):79-84 PMID: 6796764

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1910777

Published

1980-12-01

Pages

777-83

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1162277

Subset

External Links

PubMed source DOI: 10.1042/bj1910777 PMC Full Text View journal details

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Isolation and characterization of a myeloma--spleen-cell hybrid producing antibody to phenylalanine hydroxylase.

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