Preparation and partial characterization of cell-free protein-synthesizing extracts from Tetrahymena pyriformis.

The Biochemical journal ·Vol. 194 ·No. 3 ·1981-03-15 ·Pages 761-70

David ET, Smith KE

Abstract

1. We have examined methods necessary for preparing post-mitochondrial supernatants from Tetrahymena pyriformis strain HSM that are capable of efficient cell-free protein synthesis. 2. The requirements for optimum synthesis in these extracts are described. 3. Data relating to the kinetics of protein synthesis and the initiation capacity of these supernatants are presented.

MeSH Terms

Amino Acids/metabolism Cell Fractionation Cell-Free System Egtazic Acid/pharmacology Energy Metabolism Kinetics Polyribosomes/metabolism Protein Biosynthesis Tetrahymena pyriformis/metabolism

Chemicals

Amino Acids Egtazic Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

David E T

Smith K E

References (9)

9 references, click to expand

Protein synthesis in a cell-free preparation from Tetrahymena pyriformis GL.
Comp Biochem Physiol B. 1971 Dec 15;40(4):855-62 PMID: 5002922
Protein synthesis in vitro with Tetrahymena mitochondrial ribosomes.
Biochim Biophys Acta. 1972 Feb 15;259(3):369-77 PMID: 4622385
Protein synthesis in a postmitochondrial supernatant system from rat liver. An effect of diabetes at the level of peptide chain initiation.
FEBS Lett. 1973 Sep 1;35(1):169-72 PMID: 4751993
Inhibition at the initiation level of eukaryotic protein synthesis by T-2 toxin.
FEBS Lett. 1975 Jan 15;50(1):8-12 PMID: 1089073
The analysis of intermediary reactions involved in protein synthesis, in a cell-free extract of Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.
J Biol Chem. 1979 May 25;254(10):3970-6 PMID: 374405
Growth-dependent factors in the regulation of aminoacyl-tRNA synthetase activities of Tetrahymena pyriformis.
Biochim Biophys Acta. 1977 Oct 18;478(4):474-85 PMID: 410448
Structural requirements for the inhibitory action of 12,13-epoxytrichothecenes on protein synthesis in eukaryotes.
Biochem J. 1977 Sep 15;166(3):399-409 PMID: 597234
The preparation and characterization of a cell-free system from Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.
J Biol Chem. 1979 May 25;254(10):3965-9 PMID: 374404
An efficient mRNA-dependent translation system from reticulocyte lysates.
Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1940761

Published

1981-03-15

Pages

761-70

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1162811

Subset

External Links

PubMed source DOI: 10.1042/bj1940761 PMC Full Text View journal details

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Preparation and partial characterization of cell-free protein-synthesizing extracts from Tetrahymena pyriformis.

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