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PMID: 6804941 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophila gene related to the major heat shock-induced gene is transcribed at normal temperatures and not induced by heat shock.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 2 ·1982-01-00 ·Pages 525-9

Ingolia TD, Craig EA

Abstract

A gene related to the major heat shock-induced (hsp70) gene of Drosophila has been isolated from the sibling species D. melanogaster and D. simulans. This heat shock-cognate (hsc70) gene is present at cytological locus 70C. The primary sequence of approximately one-third of the protein-coding region has been compared with that of the hsp70 gene; 72% homology of the base sequence and 74% homology of the deduced amino acid sequence was found. In the codon specifying amino acid 66, the hsc70 gene contains an insertion of 1.7 kilobases; the hsp70 genes contain no intervening sequences. The sequence at the 5' and 3' junctions of the insertion is similar to that found in many intervening sequences. cDNA extension experiments indicate that the hsc70 gene is transcribed at normal temperatures in adult flies and that transcription is not enhanced by heat treatment.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Drosophila melanogaster/genetics Gene Expression Regulation Genes Heat-Shock Proteins Proteins/genetics Temperature Transcription, Genetic
Chemicals
Heat-Shock Proteins Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ingolia T D
Craig E A
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.2.525
Published
1982-01-00
Pages
525-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345776
Subset
IM
Databases
GENBANK
J01085, J01086, J01087, J01088, J01089, J01103
External Links
PubMed source DOI: 10.1073/pnas.79.2.525 PMC Full Text View journal details
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