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PMID: 6805469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Monensin inhibits synthesis of proteoglycan, but not of hyaluronate, in chondrocytes.

The Biochemical journal ·Vol. 202 ·No. 1 ·1982-01-15 ·Pages 249-54

Mitchell D, Hardingham T

Abstract

Monensin (10nm-1mum) inhibited the incorporation of [(35)S]sulphate and [(3)H]glucosamine into proteoglycans by rat chondrosarcoma cells, but the incorporation of [(3)H]glucosamine into hyaluronate was unaffected. The results suggest that hyaluronate synthesis occurs in a cell compartment separate from chondroitin sulphate synthesis.

MeSH Terms
Animals Cartilage/cytology,drug effects,metabolism Cells, Cultured Chondroitin Sulfates/biosynthesis Chondrosarcoma/metabolism Furans/pharmacology Glucosamine/metabolism Glycosides/pharmacology Hyaluronic Acid/biosynthesis Monensin/pharmacology Proteoglycans/biosynthesis Rats Sulfates/metabolism
Chemicals
Furans Glycosides Proteoglycans Sulfates 4-nitrophenyl beta-D-xyloside Hyaluronic Acid Chondroitin Sulfates Monensin Glucosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mitchell D
Hardingham T
References (12)
12 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2020249
Published
1982-01-15
Pages
249-54
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1158098
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2020249 PMC Full Text View journal details
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