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PMID: 6806250 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum.

Journal of bacteriology ·Vol. 151 ·No. 1 ·1982-07-00 ·Pages 507-9

Clark JE, Ragsdale SW, Ljungdahl LG, Wiegel J

Abstract

The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahydrofolate. These results suggest that C. thermoautotrophicum synthesizes acetate from CO2 via a formate-tetrahydrofolate-corrinoid pathway.

MeSH Terms
Acetates/metabolism Aldehyde Oxidoreductases/metabolism Carbon Dioxide/metabolism Clostridium/enzymology Formate Dehydrogenases/metabolism Hydrogenase Kinetics Multienzyme Complexes Oxidoreductases/metabolism
Chemicals
Acetates Multienzyme Complexes Carbon Dioxide Oxidoreductases Hydrogenase Formate Dehydrogenases Aldehyde Oxidoreductases carbon monoxide dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clark J E
Ragsdale S W
Ljungdahl L G
Wiegel J
References (23)
23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.1.507-509.1982
Published
1982-07-00
Pages
507-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220273
Subset
IM
Grants
NIADDK NIH HHS · AM-27323 · United States
External Links
PubMed source DOI: 10.1128/jb.151.1.507-509.1982 PMC Full Text View journal details
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