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PMID: 6808321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of DNA replication in the induction and turn-off of the SOS response in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 185 ·No. 3 ·1982-00-00 ·Pages 440-4

Casaregola S, D'Ari R, Huisman O

Abstract

We have studied the role of DNA replication in turn-on and turn-off of the SOS response in Escherichia coli using a recA::lac fusion to measure levels of recA expression. An active replication fork does not seem to be necessary for mitomycin C induced recA expression: a dnaA43 initiation defective mutant, which does not induce the SOS response at non-permissive temperature, remains mitomycin C inducible after the period of residual DNA synthesis. This induction seems to be dnaC dependent since in a dnaC325 mutant recA expression not only is not induced at 42 degrees C but becomes mitomycin C non-inducible after the period of residual synthesis. Unscheduled halts in DNA replication, generally considered the primary inducing event, are not sufficient to induce the SOS response: no increase in recA expression was observed in dnaG(Ts) mutants cultivated at non-permissive temperature. The replication fork is nonetheless involved in induction, as seen by the increased spontaneous level of recA expression in these strains at permissive temperature. Turn-off of SOS functions can be extremely rapid: induction of recA expression by thymine starvation is reversed within 10 min after restoration of normal DNA replication. We conclude that the factors involved in induction--activated RecA (protease) and the activating molecular (effector)--do not persist in the presence of normal DNA replication.

MeSH Terms
Antibiotics, Antineoplastic/pharmacology Bacterial Proteins/genetics DNA Repair DNA Replication Escherichia coli/drug effects,genetics Genotype Mitomycin Mitomycins/pharmacology Mutation Rec A Recombinases beta-Galactosidase/genetics
Chemicals
Antibiotics, Antineoplastic Bacterial Proteins Mitomycins Mitomycin Rec A Recombinases beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Casaregola S
D'Ari R
Huisman O
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39 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334136
Published
1982-00-00
Pages
440-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00334136
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