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PMID: 6809729 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Insertion of bacteriophage SP beta into the citF gene of Bacillus subtilis and specialized transduction of the ilvBC-leu genes.

Journal of bacteriology ·Vol. 151 ·No. 3 ·1982-09-00 ·Pages 1222-9

Mackey CJ, Zahler SA

Abstract

We isolated a strain of Bacillus subtilis in which the SP beta c2 prophage is inserted into the citF (succinate dehydrogenase) gene. Defective specialized transducing particles for the ilvBC-leu genes were isolated from phage-induced lysates of this lysogen. We isolated a group of phages that differ in the amount of genetic material they carry from this region. Also, we incorporated mutant ilv and leu alleles into the genomes of several transducing phages. Our phage collection enables us to identify the cistron of new ilv and leu mutations by complementation analysis. In this process we discovered a fourth leu cistron, leuD. Characterization of the phages confirmed the published gene order: ilvB-ilvC-leuA-leuC-leuB; leuD lies to the right of leuB.

MeSH Terms

Bacillus subtilis/genetics Bacteriophages/genetics Chromosome Mapping Chromosomes, Bacterial Genes Genes, Bacterial Isoleucine/biosynthesis Leucine/biosynthesis Lysogeny Succinate Dehydrogenase/genetics Transduction, Genetic Valine/biosynthesis

Chemicals

Isoleucine Succinate Dehydrogenase Leucine Valine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mackey C J

Zahler S A

References (11)

11 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.151.3.1222-1229.1982

Published

1982-09-00

Pages

1222-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC220399

Subset

Grants

NIGMS NIH HHS · GM-07617 · United States

NIGMS NIH HHS · GM-28038 · United States

External Links

PubMed source DOI: 10.1128/jb.151.3.1222-1229.1982 PMC Full Text View journal details

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Insertion of bacteriophage SP beta into the citF gene of Bacillus subtilis and specialized transduction of the ilvBC-leu genes.

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