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PMID: 6812058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of human immunoglobulin kappa light chain variable region genes to the short arm of chromosome 2 by in situ hybridization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 16 ·1982-08-00 ·Pages 4957-61

Malcolm S, Barton P, Murphy C, Ferguson-Smith MA, Bentley DL, Rabbitts TH

Abstract

The genes for human immunoglobulin kappa light chains have been localized in normal lymphocyte and fibroblast chromosomes by in situ hybridization of probes from cloned DNA fragments of the kappa variable region locus. The localization was achieved by counting grains (after autoradiography) over chromosomes in a number of karyotypes. The variable region gene probes hybridized in a cluster on a region of the chromosome 2 short arm close to the centromere (2cen leads to p12). This location was confirmed in lymphocytes from a balanced translocation carrier 46XXt (2; 16) (q13; q22). Our results show that human kappa light chain genes are located in the region of the break point observed in specific chromosomal translocations associated with Burkitt lymphoma.

MeSH Terms
Binding Sites, Antibody/genetics Cells, Cultured Chromosomes, Human, 1-3 Genes Humans Immunoglobulin Light Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin kappa-Chains/genetics Nucleic Acid Hybridization
Chemicals
Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Malcolm S
Barton P
Murphy C
Ferguson-Smith M A
Bentley D L
Rabbitts T H
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15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.16.4957
Published
1982-08-00
Pages
4957-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346804
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.16.4957 PMC Full Text View journal details
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