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PMID: 6817079 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 2 ·No. 10 ·1982-10-00 ·Pages 1212-9

Silverman SJ, Rose M, Botstein D, Fink GR

Abstract

The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient for initiating expression of beta-galactosidase activity in S. cerevisiae and confers upon this activity the regulatory response normally found for HIS4.

MeSH Terms
Base Sequence Escherichia coli Gene Expression Regulation Genetic Engineering Histidine/genetics Lac Operon Operon Saccharomyces cerevisiae/genetics Transcription, Genetic beta-Galactosidase/genetics
Chemicals
Histidine beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Silverman S J
Rose M
Botstein D
Fink G R
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21 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.10.1212-1219.1982
Published
1982-10-00
Pages
1212-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369920
Subset
IM
Grants
NCI NIH HHS · CA23441 · United States
NIGMS NIH HHS · F32 GM07161-02 · United States
NIGMS NIH HHS · GM15408 · United States
External Links
PubMed source DOI: 10.1128/mcb.2.10.1212-1219.1982 PMC Full Text View journal details
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