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PMID: 6818319 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effect of lipopolysaccharide stimulation on the transcription and translation of messenger RNA for cell surface immunoglobulin M.

The Journal of experimental medicine ·Vol. 156 ·No. 4 ·1982-10-01 ·Pages 962-74

Yuan D, Tucker PW

Abstract

Analysis of mu-specific mRNA in the B cell tumor line, BCL1, shows that the cells contain predominantly mRNA for mu chain of membrane-bound immunoglobulin M (IgM) (2.7 kb, mu m mRNA). Stimulation of the cells to Ig secretion by lipopolysaccharide (LPS) results in a 6-12 fold increase in amount of mRNA for the mu chain of secreted IgM (2.4 kb mu s mRNA). The increase in mu s mRNA is accompanied by a 3-4-fold increase in mu m mRNA. The rate of mu chain synthesis of membrane IgM in LPS-stimulated cells is, however, reduced by at least twofold, suggesting that both transcriptional and translational regulatory events are involved in the induction of B lymphocytes to secretion.

MeSH Terms
Animals Cell Line Gene Expression Regulation Immunoglobulin M/genetics Immunoglobulin mu-Chains/genetics,metabolism Lipopolysaccharides/pharmacology Mice Protein Biosynthesis RNA, Messenger/genetics Receptors, Antigen, B-Cell/genetics Transcription, Genetic
Chemicals
Immunoglobulin M Immunoglobulin mu-Chains Lipopolysaccharides RNA, Messenger Receptors, Antigen, B-Cell
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yuan D
Tucker P W
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37 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.156.4.962
Published
1982-10-01
Pages
962-74
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186812
Subset
IM
Grants
NIAID NIH HHS · AI-11851 · United States
NIAID NIH HHS · AI-16547 · United States
NCI NIH HHS · CA31534 · United States
External Links
PubMed source DOI: 10.1084/jem.156.4.962 PMC Full Text View journal details
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