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PMID: 6818555 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 24 ·1982-12-00 ·Pages 7862-5

Rice D, Baltimore D

Abstract

We have introduced a functionally rearranged murine kappa light chain immunoglobulin (Ig) gene into an Abelson murine leukemia virus-transformed lymphoid cell line. Plasmid pSV2gpt-kappa 41, containing the kappa light chain gene from the myeloma MOPC41 and the selectable marker gene gpt, was introduced into 81A-2 cells by the calcium phosphate coprecipitation technique. Cells expressing the gpt gene were selected by growth in medium containing mycophenolic acid. One transfected cell line, kappa-2, was shown to make kappa mRNA and polypeptide chains and to assemble the kappa chain product with gamma 2b heavy chains to form an apparently complete IgG2b. When bacterial lipopolysaccharide was added to the growth medium, levels of kappa mRNA and polypeptide increased, showing regulated expression of the introduced kappa gene.

MeSH Terms
Animals Cell Line Gene Expression Regulation Genes Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/biosynthesis,genetics Mice Plasmids Transcription, Genetic Transformation, Genetic
Chemicals
Immunoglobulin Light Chains Immunoglobulin kappa-Chains
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rice D
Baltimore D
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.24.7862
Published
1982-12-00
Pages
7862-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347449
Subset
IM
Grants
NCI NIH HHS · CA14051 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.24.7862 PMC Full Text View journal details
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