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PMID: 6840845 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens.

Infection and immunity ·Vol. 39 ·No. 3 ·1983-03-00 ·Pages 1428-40

Collins MT, Cho SN, Høiby N, Espersen F, Baek L, Reif JS

Abstract

By crossed immunoelectrophoresis, 85 different antigens were demonstrated in sonicated preparations of Legionella pneumophila serogroup 1 (Lp1). The precipitin patterns of 82 anodic-migrating antigens were numbered and were designated the Lp1 reference system. Eleven antigens were stable to boiling, and seven of these were shown to be surface antigens. One heat-stable surface antigen (antigen no. 61) was highly reactive with limulus amoebocyte lysates and formed a precipitin resembling lipopolysaccharide. Serum from an isolation confirmed case of Lp1 infection and serogroup-specific rabbit antiserum reacted specifically with antigen no. 61, which was designated the serogroup-specific antigen. Normal human and rabbit sera commonly had antibodies to antigen no. 66 of the Lp1 reference system. This antigen is antigenically related to the "common antigen" of Pseudomonas aeruginosa.

MeSH Terms
Animals Antibodies, Bacterial/analysis Antigens, Bacterial/analysis Antigens, Surface/analysis Hot Temperature Humans Immunoelectrophoresis, Two-Dimensional Legionella/classification,immunology Legionnaires' Disease/immunology Limulus Test Precipitin Tests Rabbits Serotyping
Chemicals
Antibodies, Bacterial Antigens, Bacterial Antigens, Surface
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Collins M T
Cho S N
Høiby N
Espersen F
Baek L
Reif J S
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41 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.39.3.1428-1440.1983
Published
1983-03-00
Pages
1428-40
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC348114
Subset
IM
Grants
NCRR NIH HHS · 5 507-RR-05458-7 · United States
External Links
PubMed source DOI: 10.1128/iai.39.3.1428-1440.1983 PMC Full Text View journal details
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