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PMID: 6854738 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transformation of rodent cells by DNA extracted from transformation-defective adenovirus mutants.

Journal of virology ·Vol. 46 ·No. 3 ·1983-06-00 ·Pages 1039-44

Rowe DT, Graham FL

Abstract

Complementation group II host range mutants of adenovirus type 5 which map in early region 1B (E1B, 4.5 to 11.0 map units) have been shown to be defective for the synthesis of the E1B 58,000-dalton (58K) antigen in infections of HeLa or KB cells (Lassam et al., Cell 18:781-791, 1979) and unable to transform cultured rodent cells (Graham et al., Virology 86:10-21, 1978). In this report we show that DNA extracted from group II mutants hr6 and hr50 can transform rat cells with the same efficiency as wild-type DNA. Furthermore, group II mutant-transformed hamster cells were shown to contain no detectable E1B 58K tumor antigen but were capable of inducing tumors in newborn hamsters. Hamster cell lines 1019-3 and 1019-C3, transformed by hr50 DNA, produced no detectable quantities of either the E1B 58K or 19K antigen but nonetheless exhibited a fully transformed oncogenic phenotype. Our results show that the E1B 58K antigen is not absolutely required for oncogenic transformation and suggest that even cells lacking the 19K protein can be oncogenic.

MeSH Terms
Adenoviruses, Human/genetics,immunology Animals Antigens, Viral/genetics Antigens, Viral, Tumor Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Cricetinae DNA, Viral Genes, Viral Mutation Rats Transfection
Chemicals
Antigens, Viral Antigens, Viral, Tumor DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rowe D T
Graham F L
References (36)
36 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  3. Oncogenic transformation of hamster embryo cells in vitro by adenovirus type 5.
    Nature. 1973 May 18;243(5403):162-3 PMID: 4706286
  4. Transformation of rat cells by DNA of human adenovirus 5.
    Virology. 1973 Aug;54(2):536-9 PMID: 4737663
  5. Size and location of the transforming region in human adenovirus type 5 DNA.
    Nature. 1974 Oct 25;251(5477):687-91 PMID: 4473721
  6. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;.
    J Mol Biol. 1974 Oct 15;89(1):49-72 PMID: 4613857
  7. Properties of temperature-sensitive mutants of polyoma virus.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:37-40 PMID: 169081
  8. Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:637-50 PMID: 169096
  9. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.
    Virology. 1977 Mar;77(1):319-29 PMID: 841862
  10. Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
    J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
  11. Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):79-83 PMID: 203944
  12. Defective transforming capacity of adenovirus type 5 host-range mutants.
    Virology. 1978 May 1;86(1):10-21 PMID: 664220
  13. Synthesis of DNA, late polypeptides, and infectious virus by host-range mutants of adenovirus 5 in nonpermissive cells.
    Virology. 1978 Jun 15;87(2):463-7 PMID: 664263
  14. Structure of the adenovirus 2 early mRNAs.
    Cell. 1978 Jul;14(3):695-711 PMID: 688389
  15. Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue.
    Virology. 1978 Nov;91(1):39-50 PMID: 726266
  16. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  17. Adenovirus 5 DNA sequences present and RNA sequences transcribed in transformed human embryo kidney cells (HEK-Ad-5 or 293).
    Virology. 1979 Apr 30;94(2):460-9 PMID: 452423
  18. Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
    Cell. 1979 Aug;17(4):935-44 PMID: 487437
  19. In vitro translation products specified by the transforming region of adenovirus type 2.
    J Virol. 1979 Sep;31(3):621-9 PMID: 513190
  20. Characterization of tumor antigens in cells transformed by fragments of adenovirus type 5 DNA.
    Virology. 1979 Dec;99(2):372-85 PMID: 516452
  21. Tumor antigens of human Ad5 in transformed cells and in cells infected with transformation-defective host-range mutants.
    Cell. 1979 Nov;18(3):781-91 PMID: 519755
  22. Identification of the adenovirus early proteins and their genomic map positions.
    Virology. 1980 Jan 30;100(2):419-32 PMID: 7352373
  23. Polyoma large tumor antigen is not required for tumorigenesis mediated by viral DNA.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1336-40 PMID: 6246489
  24. Adenovirus early region 1b gene function required for rescue of latent adeno-associated virus.
    Virology. 1980 Jul 30;104(2):502-5 PMID: 6249041
  25. Physical location of host-range mutations of adenovirus type 5; deletion and marker-rescue mapping.
    Virology. 1980 Jul 30;104(2):510-3 PMID: 6994340
  26. Coding capacity of a 35 percent fragment of the polyoma virus genome is sufficient to initiate and maintain cellular transformation.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3278-82 PMID: 6251451
  27. Predicted structure of two adenovirus tumor antigens.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3778-82 PMID: 6253988
  28. Transformation of rat embryo fibroblasts by cloned polyoma virus DNA fragments containing only part of the early region.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3978-82 PMID: 6254006
  29. Polyoma large T antigen regulates the integration of viral DNA sequences into the genome of transformed cells.
    Cell. 1981 Feb;23(2):347-55 PMID: 6258800
  30. Adenovirus early gene products may control viral mRNA accumulation and translation in vivo.
    Cell. 1981 Feb;23(2):485-96 PMID: 7471209
  31. The nucleotide sequence of the transforming early region E1 of adenovirus type 5 DNA.
    Gene. 1980 Nov;11(3-4):299-309 PMID: 6260576
  32. The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets.
    Cell. 1981 Nov;27(1 Pt 2):121-31 PMID: 7326748
  33. Integration and expression of viral DNA in cells transformed by host range mutants of adenovirus type 5.
    J Virol. 1982 Feb;41(2):674-85 PMID: 7077750
  34. Characterization of cells transformed by Ad5/Ad12 hybrid early region I plasmids.
    Virology. 1982 Jul 30;120(2):422-32 PMID: 7101731
  35. Identification of adenovirus-type 12 gene products involved in transformation and oncogenesis.
    Virology. 1982 Oct 15;122(1):15-28 PMID: 7135830
  36. Mapping of a 14,000-dalton antigen to early region 4 of the human adenovirus 5 genome.
    J Virol. 1983 Feb;45(2):514-23 PMID: 6834467
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.46.3.1039-1044.1983
Published
1983-06-00
Pages
1039-44
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256581
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.46.3.1039-1044.1983 PMC Full Text View journal details
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