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PMID: 6862630 Published · ppublish English Journal Article

Local immune response to repeated topical antigen application in the simian labial mucosa.

Infection and immunity ·Vol. 41 ·No. 1 ·1983-07-00 ·Pages 399-409

Nair PN, Schroeder HE

Abstract

Minor salivary glands of the oral mucosa in healthy monkeys (Macaca fascicularis and Macaca mulatta) contain organized structural units suitable for recognizing and processing antigens. A previous study of M. fascicularis monkeys provided experimental evidence of retrograde access of oral antigens deep into the minor salivary glands. The present study aimed at exploring the possible immune response of simian oral mucosa to repeated topical application of a chemically defined antigenic solution at the labial and gut mucosa. Ten female M. fascicularis animals were challenged topically at the lower lip mucosa at weekly intervals for a variable period of 4 to 8 weeks with a solution consisting of horseradish peroxidase, ferritin, and special India ink. Transmission electron microscopic examination of immunohistochemically treated sections of the labial glands revealed the presence of plasma cells containing specific anti-horseradish peroxidase antibody. These cells resided in the interacinar regions. Enteric and gut priming with the same antigen in four other monkeys, bypassing the oral mucosa, failed to reveal the presence of horseradish peroxidase-positive plasma cells in the labial mucosa of any of the four animals, although in one animal such cells could be identified in a mesenteric lymph node. This is suggestive of the existence, at least in primates, of a local immune response of the oral mucosa independent of systemic involvement.

MeSH Terms
Animals Antibody Formation Female Horseradish Peroxidase/immunology Immunization Intestinal Mucosa/immunology Lip Lymph Nodes/immunology Macaca fascicularis Macaca mulatta Microscopy, Electron Mouth Mucosa/immunology,ultrastructure Plasma Cells/immunology Salivary Glands/immunology,ultrastructure
Chemicals
Horseradish Peroxidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nair P N
Schroeder H E
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.41.1.399-409.1983
Published
1983-07-00
Pages
399-409
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264791
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.41.1.399-409.1983 PMC Full Text View journal details
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