Conformational analysis of the trinucleoside diphosphate 3'd(A2'-5'A2'-5'A). An NMR and CD study.
A 500 MHz and 300 MHz NMR study of the trinucleoside diphosphate 3'd(A2'-5'A2'-5'A) is presented. In addition, circular dichroism is used to study base stacking in the title compound. The complete 1H-NMR spectral assignment of the sugar ring proton signals is given. Information about the sugar ring (N- or S-type conformation) and about the backbone geometry along C4'-C5' and C5'-O5' bonds is obtained from the NMR coupling constants. It is shown that the trimer mainly occurs in the N-N-N stacked state at low temperatures; the presence of a minor amount of N-N-S conformational sequence is indicated.
12 references, click to expand
-
Conformation of the exocyclic 5'-CH 2 OH in nucleosides and nucleotides in aqueous solution from specific assignments of the H 5' and H 5'' signals in the NMR spectra.Biochem Biophys Res Commun. 1972 Aug 7;48(3):636-42 PMID: 5047692
-
Conformational analysis of the sugar ring in nucleosides and nucleotides. A new description using the concept of pseudorotation.J Am Chem Soc. 1972 Nov 15;94(23):8205-12 PMID: 5079964
-
Conformational analysis of the sugar ring in nucleosides and nucleotides. Improved method for the interpretation of proton magnetic resonance coupling constants.J Am Chem Soc. 1973 Apr 4;95(7):2333-44 PMID: 4709237
-
Proton magnetic resonance studies of 2'-,3'-, and 5'-deoxyadenosine conformations in solution.Nucleic Acids Res. 1977 Apr;4(4):939-53 PMID: 301272
-
Nature of inhibitor of cell-free protein synthesis formed in response to interferon and double-stranded RNA.Nature. 1977 Aug 11;268(5620):540-2 PMID: 196217
-
An interferon-induced phosphodiesterase degrading (2'-5') oligoisoadenylate and the C-C-A terminus of tRNA.Proc Natl Acad Sci U S A. 1979 Oct;76(10):4788-92 PMID: 228264
-
Conformational characteristics of the trinucleoside diphosphate dApdApdA and its constituents from nuclear magnetic resonance and circular dichroism studies. Extrapolation to the stacked conformers.Eur J Biochem. 1980 Nov;112(1):95-110 PMID: 6935052
-
Synthesis and characterization of (2'-5')ppp3'dA(p3'dA)n, an analogue of (2'-5')pppA(pA)n.Nature. 1981 May 28;291(5813):355-8 PMID: 7231556
-
Conformational analysis of the nucleotides A2'-5'A, A2'-5'A2'-5'A and A2'-5'U from nuclear magnetic resonance and circular dichroism studies.Eur J Biochem. 1981 May 15;116(2):403-12 PMID: 7250133
-
Complete assignment and conformational analysis of a deoxyribotetranucleotide. d(TAAT). A 360 and 500 Mhz NMR study.Biochim Biophys Acta. 1981 Sep 28;655(2):256-64 PMID: 7284386
-
Circular dichroism studies of 6-N-methylated adenylyladenosine and adenylyluridine and their parent compounds. Thermodynamics of stacking.Eur J Biochem. 1980 May;106(1):85-95 PMID: 7341235
-
Analogs of (A2'p)nA. Correlation of structure of analogs of ppp(A2'p)2A and (A2'p)2A with stability and biological activity.J Biol Chem. 1982 Nov 25;257(22):13390-7 PMID: 7142154
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
