Home LiteratureArticle Details
PMID: 687118 Published · ppublish English Journal Article

Structural elements in adenovirus cores. Studies by means of freeze-fracturing and ultrathin sectioning.

Archives of virology ·Vol. 57 ·No. 4 ·1978-00-00 ·Pages 323-37

Nermut MV

Abstract

Rod-like elements have been observed in adenovirions after freeze-fracturing of purified, semi-purified and intra-cellular adenovirus type 5. Their rod-like shape was clearly demonstrated by means of stereomicrographs. Ultrathin sections prepared in parallel with the freeze-fracture replicas revealed linear structures such as ribbons, rings or rods in up to 30 per cent of the semi-purified or intracellular virus particles. These observations indicate that the main 'structural element' in the adenovirus interior is a linear structure either fragmented into 5 to 6 'rods' or folded 5 to 6 times to fit into the inner space of the virion.

MeSH Terms
Adenoviruses, Human/ultrastructure Capsid/ultrastructure Cell Line Freeze Fracturing Frozen Sections Models, Structural Viral Proteins Virion/ultrastructure
Chemicals
Viral Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Nermut M V
References (22)
22 references, click to expand
  1. Fine structure of adenovirus type 5. I. Virus capsid.
    Virology. 1975 Jun;65(2):480-95 PMID: 48307
  2. Incomplete particles of adenovirus type 2. III. Viral and cellular DNA sequences in incomplete particles.
    Virology. 1977 Jan;76(1):365-79 PMID: 835233
  3. Structure and development of viruses as observed in the electron microscope. V. Entry and uncoating of adenovirus.
    J Virol. 1969 Nov;4(5):777-96 PMID: 5360527
  4. Freeze-fracturing of monolayers (capillary layers) of cell, membranes and viruses: some technical considerations.
    J Microsc. 1977 Jul;110(2):121-32 PMID: 335072
  5. Incomplete particles of adenovirus. II. Kinetics of formation and polypeptide composition of adenovirus type 2.
    Virology. 1974 Aug;60(2):431-7 PMID: 4844423
  6. Chromatin-like organization of the adenovirus chromosome.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):401-4 PMID: 1061143
  7. Structural proteins of adenoviruses. XII. Location and neighbor relationship among proteins of adenovirion type 2 as revealed by enzymatic iodination, immunoprecipitation and chemical cross-linking.
    Virology. 1975 Sep;67(1):197-208 PMID: 808902
  8. Electron microscopy of adenovirus cores.
    J Gen Virol. 1975 Jul;28(1):49-58 PMID: 808587
  9. Structure and composition of the adenovirus type 2 core.
    J Virol. 1975 Aug;16(2):366-87 PMID: 1152144
  10. In vitro reconstitution, hexon bonding and handedness of incomplete adenovirus capsid.
    J Mol Biol. 1974 Jan 5;85(4):617-30 PMID: 4852383
  11. The preparation and properties of adenovirus cores.
    J Gen Virol. 1971 Apr;11(1):35-46 PMID: 5576090
  12. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
    J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
  13. A simple fracturing device for obtaining complementary replicas of freeze-fractured and freeze-etched suspensions and tissue fragments.
    J Microsc. 1974 Jul;101(Pt 2):177-86 PMID: 4610139
  14. The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions.
    Virology. 1974 Feb;57(2):409-24 PMID: 4817074
  15. Aqueous glasses as matrices in freeze-fracture electron microscopy.
    Nature. 1976 Jul 22;262(5566):323-5 PMID: 958383
  16. The relationship of adenovirus-induced paracrystalline structures of the virus core protein(s).
    J Gen Virol. 1972 Mar;14(3):261-70 PMID: 4112037
  17. Intermediates in adenovirus assembly.
    J Virol. 1976 Aug;19(2):533-47 PMID: 957481
  18. Effect of fixatives on fracture plane in red blood cells.
    J Microsc. 1974 Sep;102(Pt 1):29-39 PMID: 4216636
  19. Freeze-etching observation on the nucleus of HeLa-S3 cell infected with adenovirus type 12.
    J Electron Microsc (Tokyo). 1976;25(4):297-8 PMID: 1027897
  20. Early events in the interaction of adenoviruses with HeLa cells. II. Comparative observations on the penetration of types 1, 5, 7, and 12.
    Virology. 1970 Mar;40(3):478-85 PMID: 5437782
  21. Structure and function of virion proteins of adenoviruses.
    Prog Exp Tumor Res. 1973;18:1-55 PMID: 4120865
  22. Plastic deformation during freeze-cleavage: a review.
    J Microsc. 1977 May;110(1):1-25 PMID: 333115
Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01320072
Published
1978-00-00
Pages
323-37
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01320072 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2