A spectrophotometric method for the detection of contaminant chymopapains in preparations of papain. Selective modification of one type of thiol group in the chymopapains by a two-protonic-state reagent.

The Biochemical journal ·Vol. 173 ·No. 1 ·1978-07-01 ·Pages 345-7

Baines BS, Brocklehurst K

Abstract

A simple spectrophotometric method for the detection of chymopapains A and B, contaminants in some preparations of papain (EC 3.422.2), was devised. If the amount of rapidly reacting thiol in the preparation, as determined by increase in A343 consequent on reaction with the two-protonic-state thiol titrant, 2,2' -dipyridl disulphide, is greater at PH8-9 than at pH4, contamination by the chymopapains is in dicated.

MeSH Terms

Chymopapain/analysis Disulfides Endopeptidases/analysis Kinetics Papain/isolation & purification Spectrum Analysis

Chemicals

Disulfides Endopeptidases Papain Chymopapain

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Baines B S

Brocklehurst K

References (12)

12 references, click to expand

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Crystalline papain. I. Preparation, specificity, and activation.
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Preparation and characterization of enzymes from spray-dried papaya (Carica papaya) latex [proceedings].
Biochem Soc Trans. 1978;6(1):255-8 PMID: 640180
Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.
Biochem J. 1978 May 1;171(2):385-401 PMID: 26335
Covalent chromatography. Preparation of fully active papain from dried papaya latex.
Biochem J. 1973 Jul;133(3):573-84 PMID: 4733241
A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.
Biochem J. 1975 Nov;151(2):417-32 PMID: 3168
Isolation and characterization of papaya peptidase A from commercial chymopapain.
Biochemistry. 1975 Aug 12;14(16):3695-700 PMID: 240390
Covalent chromatography by thiol-disulfide interchange.
Methods Enzymol. 1974;34:531-44 PMID: 4449470
Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.
Biochem J. 1973 May;133(1):67-80 PMID: 4721623
The activation reaction of papain.
Biochim Biophys Acta. 1967 Jul 11;139(2):430-8 PMID: 4962258
The mechanism of the activation of papain.
Biochem Biophys Res Commun. 1969 Mar 10;34(5):575-81 PMID: 5777776
Fractionation of proteolytic enzymes of dried papaya latex. Isolation and preliminary characterization of a new proteolytic enzyme.
C R Trav Lab Carlsberg. 1967;36(4):67-83 PMID: 6043136

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1730345

Published

1978-07-01

Pages

345-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1185781

Subset

External Links

PubMed source DOI: 10.1042/bj1730345 PMC Full Text View journal details

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A spectrophotometric method for the detection of contaminant chymopapains in preparations of papain. Selective modification of one type of thiol group in the chymopapains by a two-protonic-state reagent.

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