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PMID: 6885043 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting.

Human genetics ·Vol. 64 ·No. 2 ·1983-00-00 ·Pages 110-5

Müller CR, Davies KE, Cremer C, Rappold G, Gray JW, Ropers HH

Abstract

Human Y chromosomes were purified by dual beam flow sorting from a human X Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into lambda gtWES . lambda B vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified.

MeSH Terms
Animals Cloning, Molecular/methods Cricetinae Cricetulus DNA/genetics DNA, Recombinant Genes Humans Hybrid Cells/ultrastructure Male Nucleic Acid Hybridization Sex Chromosomes/ultrastructure Y Chromosome/ultrastructure
Chemicals
DNA, Recombinant DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Müller C R
Davies K E
Cremer C
Rappold G
Gray J W
Ropers H H
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36 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00327104
Published
1983-00-00
Pages
110-5
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327104 View journal details
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