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PMID: 6894712 Published · ppublish English Journal Article

Evidence in support of the P1/P2 purinoceptor hypothesis in the guinea-pig taenia coli.

British journal of pharmacology ·Vol. 73 ·No. 3 ·1981-07-00 ·Pages 617-24

Brown CM, Burnstock G

Abstract

1 The relaxations induced by adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate (AMP) and adenosine on the carbachol-contracted taenia coli of the guinea-pig have been studied. ATP and ADP produce similar responses which differ in nature and time course from those of AMP and adenosine. 2 Theophylline, at concentrations (25-200 muM) lower than those which produce significant phosphodiesterase inhibition, blocks the effects of AMP and adenosine but fails to antagonize the responses elicited by ATP and ADP. The antagonism of adenosine by theophylline appears to be competitive. 3 Apamin (1-100 nM) blocks the inhibitory effects of ATP and ADP but fails to antagonize the responses to AMP and adenosine. The antagonism by apamin is non-competitive. 4 The results indicate that ATP and adenosine relax the taenia coli by activating different receptors and are consistent with the P1, P2 purinoceptor hypothesis.

MeSH Terms
Adenine Nucleotides/pharmacology Adenosine/pharmacology Animals Apamin/pharmacology Colon/analysis,drug effects Dose-Response Relationship, Drug Female Guinea Pigs Male Muscle Contraction/drug effects Purines/metabolism Receptors, Drug/analysis Receptors, Purinergic Theophylline/pharmacology
Chemicals
Adenine Nucleotides Purines Receptors, Drug Receptors, Purinergic Apamin Theophylline Adenosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown C M
Burnstock G
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20 references, click to expand
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1981.tb16796.x
Published
1981-07-00
Pages
617-24
Language
English
Region
England
NLM ID
7502536
PMCID
PMC2071695
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1981.tb16796.x PMC Full Text View journal details
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