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PMID: 6896875 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and organization of a mammalian 5 S gene cluster.

The Journal of biological chemistry ·Vol. 257 ·No. 19 ·1982-10-10 ·Pages 11706-11

Hart RP, Folk WR

Abstract

We have cloned and characterized a G+C-rich 2.2-kilobase pair Bam HI fragment which contains a single Syrian hamster 5 S rRNA gene. The cloned fragment directs the synthesis in vitro of 5 S RNA catalyzed by RNA polymerase III. Southern hybridization analysis of genomic hamster DNA indicates that the sequences present in the clone are tandemly repeated with a redundancy of 2700 copies per diploid cell.

MeSH Terms

Animals Base Sequence Cell Line Cricetinae Cricetulus DNA/metabolism Female Genes Kidney Nucleic Acid Hybridization Oocytes/metabolism Plasmids Protein Biosynthesis RNA Polymerase III/metabolism RNA, Ribosomal/genetics Transcription, Genetic Xenopus

Chemicals

RNA, Ribosomal DNA RNA Polymerase III

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hart R P

Folk W R

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1982-10-10

Pages

11706-11

Language

English

Region

United States

NLM ID

2985121R

Subset

Grants

NIGMS NIH HHS · 2T32 GM 07315 · United States

NICHD NIH HHS · HD 13506-03 · United States

Databases

GENBANK

J00063

External Links

PubMed source View journal details

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Structure and organization of a mammalian 5 S gene cluster.

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