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PMID: 6930658 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Physical mapping of primary and secondary origins of bacteriophage T7 DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2656-60

Tamanoi F, Saito H, Richardson CC

Abstract

Deletion mutants of bacteriophage T7 have been used to identify and to map, by electron microscopy, the origins of T7 DNA replication. The primary origin of phage T7 DNA replication lies within a 100-base-pair region located 14.75-15.0% of the distance from the genetic left end of the DNA molecule. T7 phage whose DNA contains a deletion of this region initiate replication at secondary origins, the predominant one of which is located at a distance approximately 4% from the left end of the molecule.

MeSH Terms
Chromosome Deletion Chromosome Mapping DNA Replication DNA, Viral/genetics Genes, Regulator Microscopy, Electron T-Phages/genetics Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tamanoi F
Saito H
Richardson C C
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2656
Published
1980-05-00
Pages
2656-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349461
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.5.2656 PMC Full Text View journal details
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