Home LiteratureArticle Details
PMID: 6934363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translational accuracy enhanced in vitro by (p)ppGpp.

Molecular & general genetics : MGG ·Vol. 180 ·No. 1 ·1980-00-00 ·Pages 139-45

Wagner EG, Kurland CG

Abstract

The effect of (p)ppGpp on the accuracy of translation in vitro was studied with a system that has a missense error frequency similar to that of living bacteria. When poly (U) is translated, limitation of the system in Phe increases the Leu missense error frequency. The introduction of (p)ppGpp to the Phe-limited mixtures reduces significantly the missense errors as well as reduces the rate of translation. The introduction of (p)ppGpp to a full system has no effect on the accuracy of translation but does reduce its rate. The effects of (p)ppGpp on rate and accuracy of translation can be simulated in part by other inhibitors of translation such as GDPCP, fusidic acid and tetracycline. Furthermore, the presence of ppGpp or GDPCP in a Phe-limited system leads to an accumulation of Phe-tRNA, while a Phe-limited system that contains only GTP has negligibly small concentrations of Phe-tRNA. We conclude that one way in which (p)ppGpp improves the accuracy of translation is by permitting the system to maintain a favorable Phe-tRNA/Leu-tRNA ratio.

MeSH Terms
Cell-Free System Gene Frequency/drug effects Guanine Nucleotides/pharmacology Guanosine Pentaphosphate/genetics,pharmacology Guanosine Tetraphosphate/genetics In Vitro Techniques Leucine/genetics Mutation/drug effects Phenylalanine/genetics Protein Biosynthesis/drug effects RNA, Transfer/genetics
Chemicals
Guanine Nucleotides Guanosine Tetraphosphate Guanosine Pentaphosphate Phenylalanine RNA, Transfer Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wagner E G
Kurland C G
References (31)
31 references, click to expand
  1. RNA overproducing revertants of an alanyl-tRNA synthetase mutant of Escherichia coli.
    Mol Gen Genet. 1972;119(4):323-35 PMID: 4567806
  2. Two compounds implicated in the function of the RC gene of Escherichia coli.
    Nature. 1969 Mar 1;221(5183):838-41 PMID: 4885263
  3. Mistranslation in E. coli.
    Cell. 1977 Jan;10(1):131-7 PMID: 138485
  4. Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1313-7 PMID: 4624757
  5. Inhibition of in vitro protein synthesis by ppGpp.
    FEBS Lett. 1972 Oct 15;27(1):71-75 PMID: 11946810
  6. Role of guanine nucleotides in protein synthesis. Elongation factor G and guanosine 5'-triphosphate,3'-diphosphate.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3250-4 PMID: 4594040
  7. Defective translation in RC - cells.
    Nat New Biol. 1972 May 31;237(74):131-5 PMID: 4556374
  8. Interaction of guanosine 5'-diphosphate, 2'-(or 3'-) diphosphate(ppGpp) with elongation factors from E. coli.
    Biochem Biophys Res Commun. 1972 Jul 11;48(1):191-6 PMID: 4557508
  9. Suppression of temperature-sensitive aminoacyl-tRNA synthetase mutations by ribosomal mutations: a possible mechanism.
    Mol Gen Genet. 1976 Nov 24;149(1):51-61 PMID: 796671
  10. Accumulation and turnover of guanosine tetraphosphate in Escherichia coli.
    J Mol Biol. 1972 Nov 28;71(3):769-83 PMID: 4567473
  11. The suppression of defective translation by ppGpp and its role in the stringent response.
    Cell. 1978 Jul;14(3):545-57 PMID: 357011
  12. The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response.
    J Biol Chem. 1970 May 10;245(9):2309-18 PMID: 4315151
  13. A convenient assay for mono-, di-, and oligophenylalanines.
    Anal Biochem. 1969 Apr 4;28(1):375-84 PMID: 5781430
  14. Identification of the synthesis of guanosine tetraphosphate (MS I) as insertion of a pyrophosphoryl group into the 3'-position in guanosine 5'-diphosphate.
    Proc Natl Acad Sci U S A. 1973 Feb;70(2):306-9 PMID: 4346881
  15. The interaction of guanosine 5'-diphosphate, 2' (3')-diphosphate with the bacterial elongation factor Tu.
    Arch Biochem Biophys. 1973 Feb;154(2):675-82 PMID: 4570843
  16. Thermolability of the stringent factor in rel mutants of Escherichia coli.
    J Mol Biol. 1973 Jul 15;77(4):625-9 PMID: 4579452
  17. The role of guanine nucleotides in protein biosynthesis.
    Biophys J. 1978 Jun;22(3):373-92 PMID: 248284
  18. Ribosomal protein L7/L12 is required for optimal translation.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4007-10 PMID: 7001453
  19. Biochemical and genetic characterization of a mutant of Escherichia coli with a temperature-sensitive valyl ribonucleic acid synthetase.
    J Bacteriol. 1966 Oct;92(4):1076-82 PMID: 5333025
  20. Interactions of guanosine triphosphate analogues with elongation factor G of Escherichia coli.
    Eur J Biochem. 1976 Apr 1;63(2):431-40 PMID: 770173
  21. The effect of enzymatically synthesized ribonucleic acid on amino acid incorporation by a soluble protein-ribosome system from Escherichia coli.
    Proc Natl Acad Sci U S A. 1962 Jan 15;48:94-104 PMID: 14040327
  22. Inhibition of translation initiation complex formation by MS1.
    FEBS Lett. 1972 Jun 15;23(2):163-6 PMID: 4564147
  23. [New mutants for regulation of RNA synthesis].
    Bull Soc Chim Biol (Paris). 1965;47(8):1567-70 PMID: 5321960
  24. Interaction of alleles of the relA, relC and spoT genes in Escherichia coli: analysis of the interconversion of GTP, ppGpp and pppGpp.
    Mol Gen Genet. 1977 Jan 7;150(1):87-101 PMID: 319345
  25. An unusual condition of leucine transfer RNA appearing during leucine starvation of Escherichia coli.
    J Mol Biol. 1969 Jan 14;39(1):45-58 PMID: 4329287
  26. Nucleoside triphosphate regeneration decreases the frequency of translation errors.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3174-8 PMID: 290995
  27. "Two out of three" codon reading leading to mistranslation in vivo.
    Mol Gen Genet. 1980 Feb;177(3):439-45 PMID: 6768967
  28. Isolation of a lambda transducing bacteriophage carrying the relA gene of Escherichia coli.
    J Bacteriol. 1976 Aug;127(2):917-22 PMID: 783121
  29. A relaxed mutant with an altered ribosomal protein L11.
    Mol Gen Genet. 1976 Feb 27;144(1):111-4 PMID: 772409
  30. Isolation of "relaxed" mutants of Escherichia coli.
    J Bacteriol. 1968 Feb;95(2):729-31 PMID: 4867755
  31. Rapid purification of highly active ribosomes from Escherichia coli.
    Anal Biochem. 1980 Jul 1;105(2):369-74 PMID: 7006445
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267363
Published
1980-00-00
Pages
139-45
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267363
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2