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PMID: 6934535 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Quantitation of mRNAs during mouse spermatogenesis: protamine-like histone and phosphoglycerate kinase-2 mRNAs increase after meiosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 10 ·1980-10-00 ·Pages 6086-90

Erickson RP, Kramer JM, Rittenhouse J, Salkeld A

Abstract

Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate I-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography followed by two-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNAs suggest that these messages are transcribed after meiosis.

MeSH Terms
Animals Histones/biosynthesis,genetics Male Meiosis Mice Phosphoglycerate Kinase/biosynthesis,genetics Protamines/genetics RNA, Messenger/metabolism Spermatogenesis Transcription, Genetic
Chemicals
Histones Protamines RNA, Messenger Phosphoglycerate Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Erickson R P
Kramer J M
Rittenhouse J
Salkeld A
References (33)
33 references, click to expand
  1. Ribonucleic acid and protein synthesis during differentiation of male germ cells in the mouse.
    Arch Anat Microsc Morphol Exp. 1967;56(3):61-74 PMID: 5615180
  2. A catalog of sperm histones.
    Genetics. 1969;61(1):Suppl:93-111 PMID: 5345404
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. The biosynthesis of protamine during spermatogenesis of the mouse: extraction, partial characterization, and site of synthesis.
    J Cell Physiol. 1971 Aug;78(1):13-24 PMID: 5111021
  5. Amino acid analysis of stained bands from polyacrylamide gels.
    Anal Biochem. 1971 Nov;44(1):81-8 PMID: 4109030
  6. Mechanisms of meiotic drive.
    Annu Rev Genet. 1970;4:409-36 PMID: 4950062
  7. [Metabolism of ribonucleic acid and proteins in spermatocytes and spermatids of the ram (Ovis aries). I. Incorporation and fate of 3H-uridine].
    Ann Biol Anim Biochim Biophys. 1972;12(2):203-19 PMID: 4667689
  8. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  9. Initiation of globin synthesis: assays.
    Methods Enzymol. 1974;30:101-27 PMID: 4852488
  10. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  11. Gene expression and macromolecular synthesis during preimplantation embryonic development.
    Biol Reprod. 1975 Feb;12(1):82-105 PMID: 1095089
  12. Centrifugal elutriation: separation of spermatogenic cells on the basis of sedimentation velocity.
    J Cell Physiol. 1975 Aug;86(1):177-89 PMID: 1236854
  13. Ontogensis of spermatozoa autoantigens in guinea pigs.
    Differentiation. 1975 Aug 11;3(1-3):107-14 PMID: 1183756
  14. Synthesis and amino acid composition of basic proteins in mammalian sperm nuclei.
    Dev Biol. 1975 Dec;47(2):349-65 PMID: 1204939
  15. The expression of beta-glucuronidase during preimplantation development of mouse embryos.
    Dev Biol. 1976 Jan;48(1):104-9 PMID: 942653
  16. Purification and properties of biologically active rainbow trout testis protamine mRNA.
    J Biol Chem. 1976 Mar 10;251(5):1455-63 PMID: 1254578
  17. Comparative analysis of the nuclear basic proteins in rat, human, guinea pig, mouse and rabbit spermatozoa.
    Biochim Biophys Acta. 1976 Jun 15;434(2):377-89 PMID: 952894
  18. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  19. Testis specific phosphoglycerate kinase B in mouse.
    J Exp Zool. 1976 Nov;198(2):231-40 PMID: 978173
  20. Identification and isolation of protamine messenger ribonucleoprotein particles from rainbow trout testis.
    Biochemistry. 1977 Apr 5;16(7):1383-91 PMID: 849423
  21. Histone synthesis and replacement during spermatogenesis in the mouse.
    Differentiation. 1977;7(3):167-80 PMID: 323095
  22. Mouse sperm chromatin proteins: quantitative isolation and partial characterization.
    Biochemistry. 1977 Sep 6;16(18):4074-80 PMID: 911755
  23. Biochemical evidence of haploid gene activity in spermatogenesis of the mouse.
    Exp Cell Res. 1978 Jan;111(1):23-30 PMID: 620694
  24. Isolation of phosphoglycerate kinases by affinity chromatography.
    Eur J Biochem. 1978 Apr 17;85(2):493-501 PMID: 648532
  25. Protamine messenger RNA: evidence for early synthesis and accumulation during spermatogenesis in rainbow trout.
    Dev Biol. 1978 May;64(1):82-98 PMID: 658597
  26. t-Alleles and the possibility of post-meiotic gene expression during mammalian spermatogenesis.
    Fed Proc. 1978 Sep;37(11):2517-21 PMID: 357186
  27. Accumulation of the predominant pancreatic mRNAs during embryonic development.
    J Biol Chem. 1979 Mar 25;254(6):2154-9 PMID: 422575
  28. Methylmercury hydroxide enhancement of translation and transcription of ovalbumin and conalbumin mRNA's.
    J Biol Chem. 1979 Aug 25;254(16):7636-42 PMID: 89113
  29. A microsystem for the extraction and in-vitro translation of mouse embryo mRNA.
    J Reprod Fertil. 1979 May;56(1):153-8 PMID: 469836
  30. Variation among inbred strains of mice in adenosine 3':5' cyclic monophosphate levels of spermatozoa.
    Genet Res. 1979 Apr;33(2):129-36 PMID: 225240
  31. Immunological and structural relatedness of isozymes and genetic variants of 3-phosphoglycerate kinase from the mouse.
    J Biol Chem. 1980 Mar 25;255(6):2590-5 PMID: 6766938
  32. Immunological studies of the sperm-specific phosphoglycerate kinase-2 of mice.
    J Reprod Immunol. 1979 OCT;1(3):185-91 PMID: 551176
  33. HISTO-IMMUNOLOGICAL LOCALIZATION OF HYALURONIDASE IN THE BULL TESTIS.
    J Reprod Fertil. 1964 Dec;8:325-30 PMID: 14248592
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.10.6086
Published
1980-10-00
Pages
6086-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350218
Subset
IM
Grants
NIGMS NIH HHS · GM 7544 · United States
NICHD NIH HHS · HD 11738 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.10.6086 PMC Full Text View journal details
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