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PMID: 6935674 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 11 ·1980-11-00 ·Pages 6634-8

Shen CK, Maniatis T

Abstract

The relationship between DNA methylation and differential expression of rabbit beta-like globin genes was studied by using restriction enzymes that cleave the sequence C-C-G-G but are differentially inhibited by the presence of 5-methylcytosine. The methylation frequency of 13 C-C-G-G sites that flank a set of four closely linked rabbit beta-like globin genes was determined. This analysis revealed that certain sites surrounding embryonic and adult globin genes are relatively undermethylated in DNA from embryonic and adult erythroid tissues, respectively. This pattern is most pronounced for three sites that are undermethylated in erythroid cells but are totally methylated in nonerythroid cells. We conclude that the degree of CpG methylation in the rabbit beta-like globin gene cluster is correlated with gene activity, but the effect is confined to relatively small regions of DNA.

MeSH Terms

Animals Base Sequence DNA/genetics Erythropoiesis Gene Expression Regulation Genes Globins/genetics Methylation Rabbits Tissue Distribution

Chemicals

Globins DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Shen C K

Maniatis T

References (27)

27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.11.6634

Published

1980-11-00

Pages

6634-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350341

Subset

External Links

PubMed source DOI: 10.1073/pnas.77.11.6634 PMC Full Text View journal details

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Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.

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