Home LiteratureArticle Details
PMID: 6938959 Published · ppublish English Journal Article

In vitro transcription of normal, mutant, and truncated mouse alpha-globin genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 12 ·1980-12-00 ·Pages 7132-6

Talkington CA, Nishioka Y, Leder P

Abstract

We have transcribed various alpha-globin gene-containing templates by using a simplified in vitro system and have shown that the 5' end of each of the RNA polymerase II-specific transcripts corresponds to that of authentic alpha-globin mRNA. Using a truncated alpha-globin gene fragment, we then localized the promotor region of the adult alpha-globin gene to a 138-base-pair fragment of alpha-globin DNA, 141 nucleotides of which precede the initiation site. In contrast, when two naturally occurring alpha-globin gene mutants were tested as templates, no RNA polymerase II-dependent initiation was detected. Comparison of the nucleotide sequences of the normal and mutant alpha-globin genes has allowed us to focus upon nucleotide positions that might be essential for promoter activity.

MeSH Terms
Animals Cell-Free System Genes Globins/genetics HeLa Cells Mice Mutation Operon RNA Caps/genetics RNA Polymerase II/metabolism Transcription, Genetic
Chemicals
RNA Caps Globins RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Talkington C A
Nishioka Y
Leder P
References (29)
29 references, click to expand
  1. Globin composition and synthesis of hemoglobins in developing fetal mice erythroid cells.
    Science. 1967 Sep 15;157(3794):1327-9 PMID: 6039000
  2. Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.
    Proc Natl Acad Sci U S A. 1970 Nov;67(3):1468-75 PMID: 5274471
  3. Genetics of mouse hemoglobins.
    Ann N Y Acad Sci. 1974 Nov 29;241(0):25-38 PMID: 4611305
  4. Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II.
    Biochemistry. 1977 May 31;16(11):2334-43 PMID: 558798
  5. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  6. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  7. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  8. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2170-4 PMID: 209457
  9. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  10. Comparison of cloned mouse alpha- and beta-globin genes: conservation of intervening sequence locations and extragenic homology.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6187-91 PMID: 282635
  11. Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
    J Biol Chem. 1979 Jul 10;254(13):6163-73 PMID: 447704
  12. Organisation and sequences at the 5' end of a cloned complete ovalbumin gene.
    Nature. 1979 Mar 29;278(5703):428-34 PMID: 450048
  13. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
    Cell. 1979 Oct;18(2):469-84 PMID: 498279
  14. The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes.
    Cell. 1979 Nov;18(3):865-73 PMID: 519759
  15. The complete sequence of a chromosomal mouse alpha--globin gene reveals elements conserved throughout vertebrate evolution.
    Cell. 1979 Nov;18(3):875-82 PMID: 519760
  16. Distinctive nucleotide sequences of promoters recognized by RNA polymerase containing a phage-coded "sigma-like" protein.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5465-9 PMID: 118447
  17. A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
    Cell. 1980 Jan;19(1):13-25 PMID: 7357599
  18. A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
    Cell. 1980 Jan;19(1):27-35 PMID: 7357604
  19. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  20. Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
    Cell. 1980 Mar;19(3):717-28 PMID: 6153931
  21. Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6 PMID: 6929494
  22. Molecular cloning and characterization of the human beta-like globin gene cluster.
    Cell. 1980 Apr;19(4):959-72 PMID: 6155216
  23. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.
    Nature. 1980 Jun 5;285(5764):367-73 PMID: 7383157
  24. The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions.
    Cell. 1980 May;20(1):119-30 PMID: 6446404
  25. Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2806-9 PMID: 6930668
  26. DNA sequence organization of the beta-globin complex in the BALB/c mouse.
    Cell. 1980 Aug;21(1):159-68 PMID: 6250710
  27. Mouse globin system: a functional and evolutionary analysis.
    Science. 1980 Sep 19;209(4463):1336-42 PMID: 7414319
  28. Accurate transcription initiation on a purified mouse beta-globin DNA fragment in a cell-free system.
    Cell. 1980 Jul;20(3):691-9 PMID: 7418005
  29. A mouse globin gene promoter is functional in SV40.
    Cell. 1980 Oct;21(3):697-708 PMID: 6254665
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.12.7132
Published
1980-12-00
Pages
7132-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350455
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.12.7132 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2