Home LiteratureArticle Details
PMID: 6940120 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

L-Fucose-terminated glycoconjugates are recognized by pinocytosis receptors on macrophages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 2 ·1981-02-00 ·Pages 1019-22

Shepherd VL, Lee YC, Schlesinger PH, Stahl PD

Abstract

125I-Labeled L-fucose-albumin complex and rat preputial beta-glucuronidase are rapidly cleared from plasma after intravenous infusion. L-Fucose-albumin retards the plasma clearance of beta-glucuronidase whereas D-fucose-albumin is inactive. In vitro, 125I-labeled L-fucose-albumin is taken up into rat or rabbit alveolar macrophages by receptor-mediated pinocytosis. Uptake (37 degrees C) is time-dependent, is saturable with increasing ligand concentration (Kuptake = 4.4 X 10(-8) M), and requires Ca2+. 125I-labeled D-fucose-albumin is poorly taken up. Binding (4 degrees C) is saturable and Ca2+ dependent. Binding and uptake are fully inhibited by yeast mannan. A series of neoglycoproteins, including L-fucose-albumin, were tested as inhibitors of uptake of 125I-labeled beta-glucuronidase into macrophages. The following order of potency was observed: L-Fuc = D-Man greater than GlcNAc approximately D-Glc greater than D-Xyl much greater than than D-Gal = L-Ara = D-Fuc. L-Fucose-terminated oligosaccharides coupled to bovine serum albumin also block 125I-labeled beta-glucuronidase uptake into macrophages.

MeSH Terms
Animals Female Fucose Glucuronidase/metabolism Glycoproteins/pharmacology Iodine Radioisotopes Kinetics Macrophages/drug effects,physiology Oligosaccharides/pharmacology Pinocytosis/drug effects Rabbits Rats Sensory Receptor Cells/physiology Serum Albumin, Bovine
Chemicals
Glycoproteins Iodine Radioisotopes Oligosaccharides Serum Albumin, Bovine Fucose Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shepherd V L
Lee Y C
Schlesinger P H
Stahl P D
References (33)
33 references, click to expand
  1. Blood-group substances.
    Science. 1966 Apr 8;152(3719):172-81 PMID: 12325343
  2. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
  3. Beta-glucuronidase of rat liver lysosomes. Purification, properties, subunits.
    J Biol Chem. 1971 Sep 10;246(17):5398-406 PMID: 5094676
  4. Requirement for alpha-L-fucose on the macrophage membrane receptor for MIF.
    J Exp Med. 1973 Nov 1;138(5):1065-76 PMID: 4583070
  5. Beta-glucuronidase of rat preputial gland. Crystallization, properties, carbohydrate composition, and subunits.
    J Biochem. 1975 Feb;77(2):427-38 PMID: 236293
  6. Aglycosylantibody. Effects of exoglycosidase treatments on autochthonous antibody survival time in the circulation.
    J Biol Chem. 1976 Feb 25;251(4):1074-80 PMID: 55413
  7. The existence of a second route for the transfer of certain glycoproteins from the circulation into the liver.
    Biochem Biophys Res Commun. 1976 Feb 9;68(3):988-93 PMID: 1259744
  8. Comparative aspects of glycoprotein structure.
    Annu Rev Biochem. 1976;45:217-37 PMID: 786150
  9. 2-Imino-2-methoxyethyl 1-thioglycosides: new reagents for attaching sugars to proteins.
    Biochemistry. 1976 Sep 7;15(18):3956-63 PMID: 963012
  10. Attachment of thioglycosides to proteins: enhancement of liver membrane binding.
    Biochemistry. 1976 Sep 7;15(18):3963-8 PMID: 963013
  11. Effect of glycosylation on the in vivo circulating half-life of ribonuclease.
    J Biol Chem. 1976 Oct 10;251(19):6016-24 PMID: 972151
  12. Evidence for specific recognition sites mediating clearance of lysosomal enzymes in vivo.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4045-9 PMID: 186782
  13. Clearance of lysosomal hydrolases following intravenous infusion. Kinetic and competition experiments with beta-glucuronidase and N-acetyl-beta-D-glucosaminidase.
    Arch Biochem Biophys. 1976 Dec;177(2):594-605 PMID: 1015835
  14. Inhibition of the rat clearance system for agalacto-orosomucoid by yeast mannans and by mannose.
    Biochem Biophys Res Commun. 1977 Jul 11;77(1):409-15 PMID: 329842
  15. Antibodies against oligosaccharides coupled to proteins: characterization of carbohydrate specificity by radioimmune assay.
    Arch Biochem Biophys. 1978 Jan 15;185(1):61-71 PMID: 623493
  16. Evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1399-403 PMID: 274729
  17. Isolation and characterization of a mannan-binding protein from rabbit liver.
    Biochem Biophys Res Commun. 1978 Apr 14;81(3):1018-24 PMID: 666781
  18. Hepatic receptor that specifically binds oligosaccharides containing fucosyl alpha1 leads to 3 N-acetylglucosamine linkages.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2215-9 PMID: 276862
  19. The effect of alpha-mannose-terminal oligosaccharides on the survival of glycoproteins in the circulation. Rapid uptake and catabolism of bovine pancreatic ribonuclease B by nonparenchymal cells of rat liver.
    Arch Biochem Biophys. 1978 Jun;188(2):418-28 PMID: 677907
  20. The binding of d-glucosyl-neoglycoproteins to the hepatic asialoglycoprotein receptor.
    J Biol Chem. 1978 Sep 10;253(17):6107-10 PMID: 681342
  21. Human beta-glucuronidase: in vivo clearance and in vitro uptake by a glycoprotein recognition system on reticuloendothelial cells.
    Cell. 1978 Sep;15(1):269-78 PMID: 699046
  22. Plasma clearance of glycoproteins with terminal mannose and N-acetylglucosamine by liver non-parenchymal cells. Studies with beta-glucuronidase, N-acetyl-beta-D-glucosaminidase, ribonuclease B and agalacto-orosomucoid.
    Biochem J. 1978 Oct 15;176(1):103-9 PMID: 728098
  23. Unique distribution of glycoprotein receptors on parenchymal and sinusoidal cells of rat liver.
    J Biol Chem. 1979 Jun 10;254(11):4457-61 PMID: 438199
  24. Pinocytic activity of rabbit alveolar macrophages in vitro.
    J Reticuloendothel Soc. 1978 Dec;24(6):673-85 PMID: 87515
  25. An electron microscope autoradiographic study of the carbohydrate recognition systems in rat liver. I. Distribution of 125I-ligands among the liver cell types.
    J Cell Biol. 1979 Oct;83(1):47-64 PMID: 511941
  26. Receptor-mediated pinocytosis of mannose glycoconjugates by macrophages: characterization and evidence for receptor recycling.
    Cell. 1980 Jan;19(1):207-15 PMID: 6766809
  27. Carbohydrate-mediated clearance of antibody . antigen complexes from the circulation. The role of high mannose oligosaccharides in the hepatic uptake of IgM . antigen complexes.
    J Biol Chem. 1980 Mar 25;255(6):2360-5 PMID: 7358675
  28. Identification of a defence mechanism in vivo against the leakage of enterokinase into the blood.
    Biochem J. 1979 Dec 15;184(3):619-26 PMID: 395951
  29. Preparation of some new neoglycoproteins by amidination of bovine serum albumin using 2-imino-2-methoxyethyl 1-thioglycosides.
    Biochemistry. 1980 Oct 14;19(21):4899-904 PMID: 7426632
  30. Studies on the specificity of rabbit hepatic carbohydrate-binding protein using neoglycoproteins.
    Biochemistry. 1980 Oct 14;19(21):4904-8 PMID: 7426633
  31. The role of extra-hepatic tissues in the receptor-mediated plasma clearance of glycoproteins terminated by mannose or N-acetylglucosamine.
    Biochem J. 1980 Nov 15;192(2):597-606 PMID: 7236228
  32. Isolation and characterization of a mannose/N-acetylglucosamine/fucose-binding protein from rat liver.
    Biochem J. 1981 Jan 15;194(1):209-14 PMID: 7305977
  33. Changes in morphology and in lysozyme content of free alveolar cells after the intravenous injection of killed BCG in oil.
    J Reticuloendothel Soc. 1968 Feb;5(1):33-53 PMID: 5650599
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.2.1019
Published
1981-02-00
Pages
1019-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319937
Subset
IM
Grants
NCI NIH HHS · CA 12858 · United States
NIGMS NIH HHS · GM 21096 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.2.1019 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2