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PMID: 6941270 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the 26S mRNA of Sindbis virus and deduced sequence of the encoded virus structural proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2062-6

Rice CM, Strauss JH

Abstract

The nucleotide sequence of intracellular 26S mRNA of Sindbis virus has been determined by direct sequence analysis of the cDNA made to this RNA with reverse transcriptase. From this, the amino acid sequences of the encoded virus structural proteins, which include a basic capsid protein and two integral membrane glycoproteins, have been deduced. The features of these proteins as related to their functions are discussed. We suggest that three proteases are required to produce these proteins from their polyprotein precursor: a viral protease, which functions in the cytosol to release the capsid protein, signalase, which makes two cleavages to separate the glycoproteins; and a protease of the Golgi complex that cleaves after double basic residues, to process the precursor form of one of the glycoproteins.

MeSH Terms
Amino Acid Sequence Base Sequence Capsid/genetics Genes Genes, Viral Glycoproteins/genetics Membrane Proteins/genetics Nucleic Acid Conformation Protein Precursors/metabolism RNA, Messenger/genetics Sindbis Virus/genetics Viral Proteins/genetics
Chemicals
Glycoproteins Membrane Proteins Protein Precursors RNA, Messenger Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rice C M
Strauss J H
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2062
Published
1981-04-00
Pages
2062-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319283
Subset
IM
Grants
NCRR NIH HHS · 507 RR 07003 · United States
NIAID NIH HHS · AI 10793 · United States
NIGMS NIH HHS · GM 06965 · United States
Databases
GENBANK
J02363, J02364, J02365, J02366, J02367, V00073
External Links
PubMed source DOI: 10.1073/pnas.78.4.2062 PMC Full Text View journal details
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