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PMID: 6941285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mouse beta-globin and adenovirus-2 major late transcripts are initiated at the cap site in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2283-6

Hagenbüchle O, Schibler U

Abstract

Initiation of transcription on major late DNA of adenovirus serotype 2 and the beta-globin gene of the mouse was studied by using ribonucleoside [beta-32P]triphosphates as labeled precursors in vitro. The 5' termini of discrete runoff transcripts of both templates were preferentially labeled. The beta phosphate of the cap structure was labeled by [beta-32P]ATP but not [beta-32P]GTP, indicating that the beta phosphate of the cap is contributed by the initiating nucleoside triphosphate (ATP in both cases). Therefore, capping occurs on the initial nucleotide of these transcripts.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Cell-Free System Globins/genetics HeLa Cells Humans RNA Caps/metabolism Transcription, Genetic
Chemicals
RNA Caps Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hagenbüchle O
Schibler U
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2283
Published
1981-04-00
Pages
2283-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319329
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.4.2283 PMC Full Text View journal details
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