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PMID: 6946440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 4936-40

Gentz R, Langner A, Chang AC, Cohen SN, Bujard H

Abstract

Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.

MeSH Terms
Base Sequence Cloning, Molecular/methods Gene Expression Regulation Genes, Bacterial Genes, Viral Operon Plasmids T-Phages/genetics Transcription, Genetic
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gentz R
Langner A
Chang A C
Cohen S N
Bujard H
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.8.4936
Published
1981-08-00
Pages
4936-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC320300
Subset
IM
Grants
NIAID NIH HHS · AI 08619 · United States
NIGMS NIH HHS · GM 27241 · United States
Databases
GENBANK
J01759
External Links
PubMed source DOI: 10.1073/pnas.78.8.4936 PMC Full Text View journal details
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