Differentiation of mouse myeloid leukemia cells induced by 1 alpha,25-dihydroxyvitamin D3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 4990-4

Abe E, Miyaura C, Sakagami H, Takeda M, Konno K, Yamazaki T, Yoshiki S, Suda T

Abstract

Mouse myeloid leukemia cells can be induced to differentiate into macrophages in vitro by 1 alpha,25-dihydroxyvitamin D3, the active form of vitamin D3. The minimal concentration of 1 alpha,25-dihydroxyvitamin D3 to induce the cell differentiation was 0.12 nM. The degree of cell differentiation in various markers induced by 12 nM 1 alpha,25-dihydroxyvitamin D3 was nearly equivalent to that induced by 1 microM dexamethasone, the most potent known stimulator. Among several markers of the differentiation by 1 alpha,25-dihydroxyvitamin D3, phagocytic activity was induced within 24 hr, and this was followed by induction of lysozyme and locomotive activities. Similar changes were also induced by 0.01-1 microM 1 alpha-hydroxyvitamin D3. 25-Hydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3 showed only weak inducing activity. These results suggest the possibility that, in addition to its wellknown biological activities in enhancing intestinal calcium transport and bone mineral mobilization, 1 alpha, 25-dihydroxyvitamin D3 is involved in the differentiation of bone marrow cells.

MeSH Terms

Animals Calcitriol/pharmacology Cell Differentiation/drug effects Cell Division/drug effects Cell Movement/drug effects Complement C3/metabolism Dexamethasone/pharmacology Enzyme Induction/drug effects Leukemia, Experimental/pathology Macrophages/cytology Mice Muramidase/biosynthesis Phagocytosis/drug effects Receptors, Complement/metabolism Receptors, Fc/metabolism

Chemicals

Complement C3 Receptors, Complement Receptors, Fc Dexamethasone Muramidase Calcitriol

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Abe E

Miyaura C

Sakagami H

Takeda M

Konno K

Yamazaki T

Yoshiki S

Suda T

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23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.8.4990

Published

1981-08-00

Pages

4990-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC320317

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.8.4990 PMC Full Text View journal details

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Differentiation of mouse myeloid leukemia cells induced by 1 alpha,25-dihydroxyvitamin D3.

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