Home LiteratureArticle Details

PMID: 6947210 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 10 ·1981-10-00 ·Pages 5958-62

Loike JD, Silverstein SC, Sturtevant JM

Abstract

Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total heat evolved in the interval 10--37 degrees C scanned at 1 degree C min-1 ranged from 300 to 2500 X 10(-12) cal (1 Cal = 4.184 J) per cell, depending on cell density, glucose concentration, and the presence or absence of various drugs.

MeSH Terms

Animals Calorimetry, Differential Scanning Cycloheximide/pharmacology Cytochalasins/pharmacology Energy Metabolism Glucose/metabolism Glycolysis/drug effects Hot Temperature Kinetics Macrophages/drug effects,metabolism Mice Potassium Cyanide/pharmacology Sodium Fluoride/pharmacology

Chemicals

Cytochalasins Sodium Fluoride Cycloheximide Glucose Potassium Cyanide

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Loike J D

Silverstein S C

Sturtevant J M

References (11)

11 references, click to expand

Measurement of rates of phagocytosis: the use of cellular monolayers.
J Cell Biol. 1969 Jan;40(1):216-24 PMID: 4881437
Cytochalasin A and B. Inhibition of sugar uptake in cultured cells.
J Biol Chem. 1972 May 10;247(9):2964-6 PMID: 4337109
Inhibition of phagocytosis and plasma membrane mobility of the cultivated macrophage by cytochalasin B. Role of subplasmalemmal microfilaments.
J Cell Biol. 1974 Sep;62(3):647-59 PMID: 4368822
L-lactate transport in Ehrlich ascites-tumour cells.
Biochem J. 1976 Feb 15;154(2):405-14 PMID: 7237
Calorimetry as an analytical tool in biochemistry and biology.
Methods Biochem Anal. 1976;23(0):1-159 PMID: 12445
The enthalpy change on adenosine triphosphate hydrolysis. I.
J Biol Chem. 1955 Dec;217(2):603-6 PMID: 13271421
The biochemical basis of phagocytosis. IV. Some aspects of carbohydrate metabolism during phagocytosis.
Biochemistry. 1962 Jan;1:159-66 PMID: 13891026
FLUORIDE INHIBITION OF SODIUM EXTRUSION FROM SWINE ERYTHROCYTES AND ITS METABOLIS CORRELATES.
Arch Biochem Biophys. 1964 Jul 20;106:57-64 PMID: 14217205
THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY.
J Exp Med. 1965 Jan 1;121:153-70 PMID: 14253481
CYTOTOXIC TEST FOR DEMONSTRATION OF MOUSE ANTIBODY.
Methods Med Res. 1964;10:39-47 PMID: 14284941
Increased ATP and creatine phosphate turnover in phagocytosing mouse peritoneal macrophages.
J Biol Chem. 1979 Oct 10;254(19):9558-64 PMID: 489550

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.10.5958

Published

1981-10-00

Pages

5958-62

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348956

Subset

Grants

NIAID NIH HHS · AI-08697 · United States

NCI NIH HHS · CA-05724 · United States

NIGMS NIH HHS · GM-04725 · United States

External Links

PubMed source DOI: 10.1073/pnas.78.10.5958 PMC Full Text View journal details

Analysis Services

Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages.

Contact

WeChat Official Account