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PMID: 6950425 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 3 ·1982-02-00 ·Pages 754-7

Zackroff RV, Idler WW, Steinert PM, Goldman RD

Abstract

Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (approximatley 0.05-micrometer) approximatley 10 nm-diameter filaments. These results demonstrate that the triplet polypeptides are components of neural IF and that the 68,000-dalton polypeptide is an IF structural protein.

MeSH Terms
Animals Cattle Cell-Free System Cytoskeleton/metabolism,ultrastructure Microscopy, Electron Molecular Weight Protein Binding Spinal Cord
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zackroff R V
Idler W W
Steinert P M
Goldman R D
References (19)
19 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Fibrillar proteins from squid axons. I. Neurofilament protein.
    J Mol Biol. 1970 Sep 28;52(3):415-28 PMID: 5492289
  3. Neurofilament disguise, destruction and discipline.
    Nature. 1975 Aug 14;256(5518):586-9 PMID: 170526
  4. Bovine epidermal keratin filament assembly in vitro.
    Biochem Biophys Res Commun. 1976 May 3;70(1):221-7 PMID: 1275938
  5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  6. Self-assembly of bovine epidermal keratin filaments in vitro.
    J Mol Biol. 1976 Dec 15;108(3):547-67 PMID: 1018318
  7. Neurofilament proteins of rat peripheral nerve and spinal cord.
    J Cell Biol. 1978 Sep;78(3):653-62 PMID: 701353
  8. Intermediate filaments in nervous tissues.
    J Cell Biol. 1978 Dec;79(3):637-45 PMID: 83322
  9. Calcium-dependent alterations of neurofilament proteins of rat peripheral nerve.
    J Neurochem. 1979 Jan;32(1):211-9 PMID: 759574
  10. A micromethod for complete removal of dodecyl sulfate from proteins by ion-pair extraction.
    Anal Biochem. 1979 Feb;93(1):153-7 PMID: 434458
  11. Neurofilaments.
    J Neurochem. 1979 Jul;33(1):5-13 PMID: 379281
  12. In vitro assembly of intermediate filaments from baby hamster kidney (BHK-21) cells.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6226-30 PMID: 293716
  13. Formation of 100 A filaments from purified glial fibrillary acidic protein in vitro.
    J Mol Biol. 1979 Nov 25;135(1):53-68 PMID: 43400
  14. Intermediate filaments from bovine, rat, and human CNS: mapping analysis of the major proteins.
    J Neurochem. 1980 May;34(5):1149-59 PMID: 7189549
  15. In vitro reassembly of squid brain intermediate filaments (neurofilaments): purification by assembly-disassembly.
    Science. 1980 Jun 6;208(4448):1152-5 PMID: 7189605
  16. Desmin from avian smooth muscle. Purification and partial characterization.
    J Biol Chem. 1980 Jul 25;255(14):6981-9 PMID: 6893048
  17. Intermediate filaments of baby hamster kidney (BHK-21) cells and bovine epidermal keratinocytes have similar ultrastructures and subunit domain structures.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4534-8 PMID: 6159631
  18. Study of the 10-nm-filament fraction isolated during the standard microtubule preparation.
    Biochem J. 1980 Nov 1;191(2):543-6 PMID: 7195199
  19. Antibody decoration of neurofilaments.
    J Cell Biol. 1981 May;89(2):198-205 PMID: 6788775
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.3.754
Published
1982-02-00
Pages
754-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345830
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.3.754 PMC Full Text View journal details
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