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PMID: 6952228 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of the B--Z transition in poly(dG-m5dC) . poly(dG-m5dC) on nucleosome formation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1771-5

Nickol J, Behe M, Felsenfeld G

Abstract

We have studied the properties of complexes formed between histones and the methylated synthetic polydeoxynucleotide poly(dG-m5dC). poly(dG-m5dC). This polymer undergoes the transition from B DNA to left-handed Z DNA at moderate ionic strength. When the polymer is in the Z form it will bind histones, but nucleosomes are not detected. When the polymer in the B form is combined with equimolar quantities of the four core histones and digested with micrococcal nuclease, particles are formed which behave in all respects as normal nucleosome cores. When these core particles are placed in solvents that would result in conversion of the protein-free polymer to the Z form, no transition is observed. The formation of a nucleosome core particle thus stabilizes the B form, whereas the presence of the Z form prevents nucleosome formation. The results suggest that if Z DNA is present in eukaryotic nuclei, it will serve to disrupt the normal chromatin structure.

MeSH Terms
DNA Histones/metabolism Nucleic Acid Conformation Nucleosomes/ultrastructure Protein Binding Structure-Activity Relationship
Chemicals
Histones Nucleosomes DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nickol J
Behe M
Felsenfeld G
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1771
Published
1982-03-00
Pages
1771-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346062
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.6.1771 PMC Full Text View journal details
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