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PMID: 6957862 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Calcium/phospholipid regulates phosphorylation of a Mr "87k" substrate protein in brain synaptosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5249-53

Wu WC, Walaas SI, Nairn AC, Greengard P

Abstract

Depolarization-induced calcium influx into rat cerebral cortex synaptosomes increased the phosphorylation of several synaptosomal proteins as examined by 32Pi incorporation. A phosphopeptide mapping technique involving NaDodSO4/polyacrylamide gels has been used to show that phosphorylation of a Mr 87,000 substrate protein is stimulated by depolarization-induced calcium influx. Phosphorylation of this Mr 87,000 substrate occurred in synaptosomal cytosol and was markedly stimulated by calcium/phosphatidylserine. Calmodulin inhibited this phosphorylation reaction. This substrate for calcium/phospholipid-dependent protein kinase is enriched in and appears to be specific to neurons.

MeSH Terms
Animals Biological Transport, Active Calcium/metabolism Calmodulin/pharmacology Cerebral Cortex/metabolism Cytosol/metabolism Mitochondria/metabolism Molecular Weight Nerve Tissue Proteins/metabolism Phosphatidylserines/pharmacology Phosphopeptides/analysis Phosphorylation Rats Synaptosomes/drug effects,metabolism Veratridine/pharmacology
Chemicals
Calmodulin Nerve Tissue Proteins Phosphatidylserines Phosphopeptides Veratridine Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wu W C
Walaas S I
Nairn A C
Greengard P
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.17.5249
Published
1982-09-00
Pages
5249-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346873
Subset
IM
Grants
NIMH NIH HHS · MH-17387 · United States
NINDS NIH HHS · NS-08440 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.17.5249 PMC Full Text View journal details
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