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PMID: 6957866 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dependence of locally measured cellular deformability on position on the cell, temperature, and cytochalasin B.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5327-31

Petersen NO, McConnaughey WB, Elson EL

Abstract

We describe an approach to exploring cell surface-cytoskeleton interactions through direct measurements of the mechanical resistance of living cells to locally applied forces. These measurements are sensitive to variations in structure across the cell and at various depths below its surface. We find that local cellular deformability depends on the temperature and on the integrity of the cytoskeleton. Cytochalasin B increases the deformability of all regions of the cell except the nucleus.

MeSH Terms
Animals Cell Membrane/physiology,ultrastructure Cells, Cultured Cytochalasin B/pharmacology Fibroblasts/physiology,ultrastructure Kinetics Mice Temperature
Chemicals
Cytochalasin B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Petersen N O
McConnaughey W B
Elson E L
References (12)
12 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.17.5327
Published
1982-09-00
Pages
5327-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346889
Subset
IM
Grants
NIGMS NIH HHS · 2 TB2 GM 07067-06 · United States
NIGMS NIH HHS · GM 21661 · United States
NIGMS NIH HHS · GM 27160 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.17.5327 PMC Full Text View journal details
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