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PMID: 6958982 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes.

Nature ·Vol. 300 ·No. 5893 ·1982-12-16 ·Pages 611-5

Palmiter RD, Brinster RL, Hammer RE, Trumbauer ME, Rosenfeld MG, Birnberg NC, Evans RM

Abstract

A DNA fragment containing the promoter of the mouse metallothionein-I gene fused to the structural gene of rat growth hormone was microinjected into the pronuclei of fertilized mouse eggs. Of 21 mice that developed from these eggs, seven carried the fusion gene and six of these grew significantly larger than their littermates. Several of these transgenic mice had extraordinarily high levels of the fusion mRNA in their liver and growth hormone in their serum. This approach has implications for studying the biological effects of growth hormone, as a way to accelerate animal growth, as a model for gigantism, as a means of correcting genetic disease, and as a method of farming valuable gene products.

MeSH Terms
Animals Gene Expression Regulation Genetic Engineering Growth Hormone/blood,genetics Liver/physiology Metalloproteins/genetics Metallothionein/genetics Mice/growth & development Operon
Chemicals
Metalloproteins Growth Hormone Metallothionein
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Palmiter R D
Brinster R L
Hammer R E
Trumbauer M E
Rosenfeld M G
Birnberg N C
Evans R M
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
0028-0836
DOI
10.1038/300611a0
Published
1982-12-16
Pages
611-5
Language
English
Region
England
NLM ID
0410462
PMCID
PMC4881848
Subset
IM
Grants
NICHD NIH HHS · HD-15477 · United States
NICHD NIH HHS · R01 HD009172 · United States
NCI NIH HHS · T32 CA009370 · United States
NICHD NIH HHS · R01 HD017321 · United States
NIGMS NIH HHS · GM-26444 · United States
External Links
PubMed source DOI: 10.1038/300611a0 PMC Full Text View journal details
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