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PMID: 6965798 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization of mitochondria in living cells with rhodamine 123.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 2 ·1980-02-00 ·Pages 990-4

Johnson LV, Walsh ML, Chen LB

Abstract

The laser dye rhodamine 123 is shown to be a specific probe for the localization of mitochondria in living cells. By virtue of its selectivity for mitochondria and its fluorescent properties, the detectability of mitochondria stained with rhodamine 123 is significantly improved over that provided by conventional light microscopic techniques. With the use of rhodamine 123, it is possible to detect alterations in mitochondrial distribution following transformation by Rous sarcoma virus and changes in the shape and organization of mitochondria induced by colchicine treatment.

MeSH Terms
Animals B-Lymphocytes/ultrastructure Cell Survival Cells, Cultured/ultrastructure Erythrocytes/ultrastructure Gerbillinae Kidney/ultrastructure Lasers Microscopy, Fluorescence Mitochondria/ultrastructure Myocardium/ultrastructure Rhodamines Xanthenes
Chemicals
Rhodamines Xanthenes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Johnson L V
Walsh M L
Chen L B
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.2.990
Published
1980-02-00
Pages
990-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348409
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.2.990 PMC Full Text View journal details
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