A computer-assisted spectrophotometric enzyme assay. A method for the calculation of Km and V from a single reaction progress curve by the integrated form of the Lineweaver-Burke plot with particular reference to beta-lactamase.

The Biochemical journal ·Vol. 185 ·No. 1 ·1980-01-01 ·Pages 186-8

Fukagawa Y

Abstract

暂无摘要

MeSH Terms

Bacillus/enzymology Computers Hydrolysis Kinetics Methods Penicillin G Spectrophotometry, Ultraviolet beta-Lactamases

Chemicals

beta-Lactamases Penicillin G

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Fukagawa Y

References (4)

4 references, click to expand

A spectrophotometric assay of beta-lactamase action on penicillins.
Biochem J. 1974 Jun;139(3):789-90 PMID: 4368359
A direct spectrophotometric assay and determination of Michaelis constants for the beta-lactamase reaction.
Anal Biochem. 1975 Jan;63(1):17-26 PMID: 803320
A simple method for calculating Km and V from a single enzyme reaction progress curve.
Biochim Biophys Acta. 1977 Jan 11;480(1):1-13 PMID: 831830
Inhibition of beta-lactamase of Bacillus licheniformis 749/C by compound PS-5, a new beta-lactam antibiotic.
Biochem J. 1980 Jan 1;185(1):177-85 PMID: 6246876

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

Published

1980-01-01

Pages

186-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161282

Subset

External Links

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