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PMID: 6967482 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Assessment of Rhodopseudomonas sphaeroides chromatophore membrane asymmetry through bilateral antiserum adsorption studies.

Journal of bacteriology ·Vol. 143 ·No. 1 ·1980-07-00 ·Pages 221-30

Collins ML, Mallon DE, Niederman RA

Abstract

The asymmetric structure of the Rhodopseudomonas sphaeroides chromatophore membrane was examined in detail by crossed immunoelectrophoresis techniques. Because these methods are quantitative and allow increased resolution and sensitivity, it was possible to analyze simultaneously the relative transmembrane distribution of a number of previously identified antigenic components. This was demonstrated by analysis of immunoglobulin samples that were adsorbed by preincubation with either isolated chromatophores or osmotically protected spheroplasts. The photochemical reaction center, the light-harvesting bacteriochlorophyll a-protein complex, the L-lactate dehydrogenase, and reduced nicotinamide adenine dinucleotide dehydrogenase (EC 1.6.99.3) were found to be exposed on the chromatophore surface (cytoplasmic aspect of the membrane within the cell). Other antigenic components were found to be exposed on the surface of spheroplasts (periplasmic aspect of the in vivo chromatophore membrane). Antigens with determinants expressed on both sides of the chromatophore membrane were also identified. Charge shift crossed immunoelectrophoresis confirmed the suggested amphiphilic character of the pigment-protein complexes and identified several additional amphiphilic membrane components.

MeSH Terms
Bacterial Chromatophores/analysis,enzymology Bacterial Proteins/analysis Bacteriochlorophylls/analysis Immunoelectrophoresis, Two-Dimensional L-Lactate Dehydrogenase/analysis Membrane Proteins/analysis NADH, NADPH Oxidoreductases/analysis Rhodobacter sphaeroides/analysis,ultrastructure
Chemicals
Bacterial Proteins Bacteriochlorophylls Membrane Proteins L-Lactate Dehydrogenase NADH, NADPH Oxidoreductases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Collins M L
Mallon D E
Niederman R A
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31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.1.221-230.1980
Published
1980-07-00
Pages
221-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294215
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.143.1.221-230.1980 PMC Full Text View journal details
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