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PMID: 6968913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Development and characterization of allospecific long-term human cytolytic T-cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5432-6

Reiss CS, Hemler ME, Englehard VH, Mier JW, Strominger JL, Burakoff SJ

Abstract

Two long-term human cytolytic T lymphocyte (CTL) lines (VE and JR), whose cytolytic activity was dependent upon both irradiated JY cells (the stimulating alloantigen) and T-cell growth factor, were established. These lines were monitored in culture for 6-8 months. Both lines were specific for HLA-A or B antigens or both and the JR line was allospecific for HLA-B7. These CTL lines killed specific target cells at an effector-to-target ratio of 0.4 (VE) or 0.08 (JR). All of the cells, which grow in suspension, rosetted with sheep erythrocytes and reacted with an antiserum specific for human T cells. The CTL line VE was used to raise rabbit antisera that immunoprecipitated two specific polypeptides (78,000 and 33,000 daltons) from labeled membranes of these CTL lines.

MeSH Terms
Animals Blood Cell Line Cytotoxicity, Immunologic Growth Substances/pharmacology Humans Lymphocyte Activation Mice Precipitin Tests T-Lymphocytes/drug effects,immunology
Chemicals
Growth Substances
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Reiss C S
Hemler M E
Englehard V H
Mier J W
Strominger J L
Burakoff S J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.9.5432
Published
1980-09-00
Pages
5432-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350073
Subset
IM
Grants
NIAID NIH HHS · AI-10736 · United States
NIAID NIH HHS · AI-15669 · United States
NCI NIH HHS · CA 14723 · United States
External Links
PubMed source DOI: 10.1073/pnas.77.9.5432 PMC Full Text View journal details
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