Home LiteratureArticle Details
PMID: 6973066 Published · ppublish English Journal Article

Nuclear skeleton structures in some normal and tumor cells.

Molecular biology reports ·Vol. 7 ·No. 1-3 ·1981-05-22 ·Pages 139-48

Zbarsky IB

Abstract

Nuclear protein fractions, described earlier, were identified as constituents of the nuclear sap (the 'globulin fraction'), that of the nucleoli and ribonucleoprotein network (the 'acidic protein'), and of the nuclear envelope (the 'residual protein'). The latter two fractions compose the protein skeleton of the cell nucleus. An essential difference between electrophoretic profiles of nuclear skeleton structures in experimental tumors and those of normal tissues was revealed. Tumor preparations contained more high molecular weight polypeptides and, in earlier stages of growth, low molecular weight components as well. Fractionation of the nuclear matrix proteins showed that the bulk of them are soluble in diluted alkali. The alkali-insoluble fraction retains the shape of the nucleus and appears in the electron microscope as a spongy nuclear skeleton. A finely dispersed fraction sedimenting from the alkaline suspension is enriched with the pore complexes. The fractions obtained differ in protein composition and probably contain protein components which are similar in molecular weights but non-identical.

MeSH Terms
Animals Cell Nucleus/analysis Cytoskeleton/ultrastructure Female Liver/anatomy & histology Liver Neoplasms, Experimental/metabolism Microscopy, Electron Molecular Weight Nucleoproteins/analysis,biosynthesis Oocytes/analysis Rana temporaria Rats Ribonucleoproteins/analysis
Chemicals
Nucleoproteins Ribonucleoproteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Zbarsky I B
References (34)
34 references, click to expand
  1. Rat liver nuclear skeleton and ribonucleoprotein complexes containing HnRNA.
    J Cell Biol. 1978 Mar;76(3):675-91 PMID: 416034
  2. [IMPORTANCE OF BIOCHEMICAL PROPERTIES OF THE NUCLEAR APPARATUS OF THE TUMOR CELL IN THE PATHOGENESIS OF CANCER].
    Vestn Akad Med Nauk SSSR. 1965;20:3-10 PMID: 14302407
  3. [Protein composition and electron microscopy of the residual protein fractions of cell nuclei of certain normal organs and tumors of rats].
    Vopr Med Khim. 1980 May-Jun;26(3):406-10 PMID: 7456374
  4. Identification of a nuclear protein matrix.
    Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410-7 PMID: 4214419
  5. [Nuclear pore density in rat liver cells during regeneration and x-ray irradiation of the animals].
    Tsitologiia. 1979 Aug;21(8):875-81 PMID: 494390
  6. Some features of cytochemistry of tumour cell nuclei.
    Acta Unio Int Contra Cancrum. 1962;18:123-6 PMID: 14009956
  7. Metaphase chromosome structure: the role of nonhistone proteins.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:351-60 PMID: 277351
  8. A fixed site of DNA replication in eucaryotic cells.
    Cell. 1980 Feb;19(2):527-36 PMID: 7357619
  9. The nuclear protein matrix: isolation, structure, and functions.
    Adv Enzyme Regul. 1976;14:63-100 PMID: 970274
  10. Labelling of histones and nonhistones in lung nuclear matrix and chromatin fractions.
    Hoppe Seylers Z Physiol Chem. 1977 Sep;358(9):1125-31 PMID: 924385
  11. [Fractionation and biosynthesis of rat liver and Zajdela hepatoma nuclear matrix proteins].
    Biokhimiia. 1980 Aug;45(8):1417-24 PMID: 7236794
  12. The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.
    Int Rev Cytol Suppl. 1977;(6):75-186 PMID: 348630
  13. The major polypeptides of the nuclear pore complex.
    Exp Cell Res. 1978 Oct 1;116(1):85-102 PMID: 568072
  14. Heterogeneous nuclear RNA-protein fibers in chromatin-depleted nuclei.
    J Cell Biol. 1978 Sep;78(3):663-74 PMID: 701354
  15. Nuclear matrix. Isolation and characterization of a framework structure from rat liver nuclei.
    J Cell Biol. 1977 Jun;73(3):616-37 PMID: 873992
  16. [Fraction of the nuclear matrix of rat liver].
    Dokl Akad Nauk SSSR. 1980;250(6):1470-3 PMID: 7379688
  17. Nuclear envelope of Chinese hamster ovary cells. Re-formation of the nuclear envelope following mitosis.
    Biochemistry. 1980 Jan 22;19(2):277-89 PMID: 7352987
  18. Nuclear protein matrix: association with newly synthesized DNA.
    Science. 1975 Jul 25;189(4199):291-3 PMID: 1145202
  19. On the structural organization of nucleolo-chromosomal ribonucleoproteins.
    Exp Cell Res. 1962 Sep;27:570-2 PMID: 13947251
  20. Ultrastructural aspects of the ribonucleo-protein network in nuclei of Walker tumor and rat liver.
    Exp Cell Res. 1967 Apr;46(1):65-77 PMID: 5337758
  21. [Biochemical characteristics of the tumor cell].
    Usp Sovrem Biol. 1961 Sep-Oct;52:164-80 PMID: 14040433
  22. Studies on the chemical composition of nuclei and cytoplasmic granules.
    Arch Biochem Biophys. 1955 Jan;54(1):24-37 PMID: 13229354
  23. Effect of protease inhibitors on matrix proteins and the association of replicating DNA.
    Exp Cell Res. 1979 Oct 15;123(2):411-4 PMID: 499368
  24. Cytological characteristics of protein and nucleoprotein fractions of cell nuclei.
    Biochim Biophys Acta. 1959 Mar;32(1):301-2 PMID: 13628763
  25. Isolation of a nuclear ribonucleoprotein network that contains heterogeneous RNA and is bound to the nuclear envelope.
    Biochemistry. 1975 Aug 26;14(17):3808-16 PMID: 1174505
  26. STUDIES ON ACIDIC NUCLEAR PROTEINS OF THE WALKER TUMOR AND LIVER.
    Cancer Res. 1963 Sep;23:1153-63 PMID: 14070370
  27. Ultrastructure of late oocyte nuclei in Rana temporaria.
    J Cell Sci. 1977 Dec;28:1-13 PMID: 304451
  28. [Quantitative content of protein fractions in cell nuclei of normal tissue and in malignant tumors].
    Biokhimiia. 1951 Jul-Aug;16(4):314-20 PMID: 14869329
  29. Nuclear proteins. III. The fibrillar nature of the nuclear matrix.
    Exp Cell Res. 1976 Dec;103(2):341-60 PMID: 1033834
  30. On the structure of tumor cell nuclei.
    Exp Cell Res. 1962 Sep;27:573-6 PMID: 14003334
  31. [Modification of proteins in cell nuclei in growth and development of experimental tumors].
    Vopr Onkol. 1955;1(1):46-52 PMID: 13299747
  32. STUDIES ON THE COMPOSITION OF NUCLEAR RESIDUAL PROTEINS FROM RAT LIVER AND WALKER 256 CARCINOSARCOMA.
    Exp Cell Res. 1964 Jan;33:68-73 PMID: 14109154
  33. [Quantitative interrelationship between protein and nucleoprotein fractions of cell nuclei of various tissues].
    Biokhimiia. 1960 Mar-Apr;25:318-22 PMID: 13827372
  34. [Karyosphere of the oocytes of the common frog, Rana temporaria].
    Ontogenez. 1979;10(5):502-6 PMID: 315042
Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00778745
Published
1981-05-22
Pages
139-48
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00778745 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2