Home LiteratureArticle Details

PMID: 6975621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mechanism of action of porphobilinogen deaminase. The participation of stable enzyme substrate covalent intermediates between porphobilinogen and the porphobilinogen deaminase from Rhodopseudomonas spheroides.

The Biochemical journal ·Vol. 195 ·No. 1 ·1981-04-01 ·Pages 177-81

Jordan PM, Berry A

Abstract

Highly stable labelled complexes are formed between porphobilinogen deaminase and stoicheiometric amounts of [14C]porphobilinogen. On completion of the catalytic cycle by the addition of excess of substrate, the complexes yield labelled product and display all the properties expected from covalently bound enzyme intermediates involved in the deaminase catalytic sequence.

MeSH Terms

Ammonia-Lyases/metabolism Chemical Phenomena Chemistry Chromatography, Gel Hydroxylamines/pharmacology Hydroxymethylbilane Synthase/metabolism Macromolecular Substances Porphobilinogen/metabolism,pharmacology Pyrroles/pharmacology Rhodobacter sphaeroides/enzymology Sodium Dodecyl Sulfate/pharmacology

Chemicals

Hydroxylamines Macromolecular Substances Pyrroles Sodium Dodecyl Sulfate Porphobilinogen Hydroxymethylbilane Synthase Ammonia-Lyases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jordan P M

Berry A

References (13)

13 references, click to expand

The enzymatic synthesis of porphyrins from porphobilinogen. II. Uroporphyrin III.
J Biol Chem. 1958 Aug;233(2):510-5 PMID: 13563529
The enzymatic synthesis of porphyrins from porphobilinogen. III. Uroporphyrinogens as intermediates.
J Biol Chem. 1958 Aug;233(2):516-9 PMID: 13563530
The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides.
Biochem J. 1956 Jan;62(1):78-93 PMID: 13293156
Adaptation to form bacteriochlorophyll in Rhodopseudomonas spheroides: changes in activity of enzymes concerned in pyrrole synthesis.
Biochem J. 1959 Jul;72:508-18 PMID: 14414331
A tetrapyrrylmethane intermediate in the enzymatic synthesis of uroporphyrinogen.
Biochemistry. 1972 Feb 29;11(5):904-10 PMID: 5059897
Purification and properties of uroporphyrinogen I synthetase from Rhodopseudomonas spheroides.
J Biol Chem. 1973 Feb 10;248(3):1019-24 PMID: 4539746
The enzymatic synthesis of porphyrins from porphobilinogen. I. Uroporphyrin I.
J Biol Chem. 1958 Aug;233(2):501-9 PMID: 13563528
The use of non-ionic industrial detergents in liquid scintillation counting.
Lab Pract. 1975 Nov;24(11):739-40 PMID: 1207059
Biosynthesis of uroporphyrinogens from porphobilinogen: mechanism and the nature of the process.
Philos Trans R Soc Lond B Biol Sci. 1976 Feb 5;273(924):137-60 PMID: 4834
The biosynthesis of uroporphyrinogen III: order of assembly of the four porphobilinogen molecules in the formation of the tetrapyrrole ring.
FEBS Lett. 1979 Aug 15;104(2):364-6 PMID: 314389
Purification and properties of uroporphyrinogen I synthase from human erythrocytes. Identification of stable enzyme-substrate intermediates.
J Biol Chem. 1980 Mar 10;255(5):1993-9 PMID: 7354069
13C NMR as a probe for the study of enzyme-catalysed reactions: mechanism of action of 5-aminolevulinic acid dehydratase.
FEBS Lett. 1980 Jun 2;114(2):283-6 PMID: 6967019
Polypyrroles formed from porphobilinogen and amines by uroporphyrinogen synthetase of Rhodopseudomonas spheroides.
Biochem J. 1973 Jul;133(3):471-92 PMID: 4542566

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1950177

Published

1981-04-01

Pages

177-81

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1162869

Subset

External Links

PubMed source DOI: 10.1042/bj1950177 PMC Full Text View journal details

Analysis Services

Mechanism of action of porphobilinogen deaminase. The participation of stable enzyme substrate covalent intermediates between porphobilinogen and the porphobilinogen deaminase from Rhodopseudomonas spheroides.

Contact

WeChat Official Account