Home LiteratureArticle Details
PMID: 6980257 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Primary in vitro cytotoxic T cell response to non-major histocompatibility complex alloantigens in normal mice.

The Journal of experimental medicine ·Vol. 156 ·No. 2 ·1982-08-01 ·Pages 610-21

Macphail S, Yron I, Stutman O

Abstract

We have shown for the first time that it is possible to consistently generate a primary in vitro cytotoxic T cell (Tc) response to non-major histocompatibility complex alloantigens using responder cells from a normal mouse strain. This was achieved by carrying out, in the generating phase, a limiting dilution procedure in which it appears that suppressor cells that inhibit Tc activation or expansion are too dilute to manifest their effect. Moreover, the response was observed in mouse serum-(MS) as well as fetal calf serum- (FCS) supplemented media, an important finding in the light of the anomalous nonspecific effects induced by FCS. The cytotoxic response produced in MS-supplemented media was shown to be highly specific in both the generating and effector phases, whereas the responses in FCS had a strong nonspecific component.

MeSH Terms
Animals Crosses, Genetic Cytotoxicity, Immunologic Female Immunogenetics Isoantigens/genetics Male Mice Mice, Inbred BALB C/immunology Mice, Inbred CBA/immunology Mice, Inbred DBA/immunology Mice, Inbred NZB Mice, Inbred Strains/immunology Species Specificity T-Lymphocytes/immunology
Chemicals
Isoantigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Macphail S
Yron I
Stutman O
References (26)
26 references, click to expand
  1. A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented media.
    J Immunol Methods. 1973 Oct;3(2):147-63 PMID: 4271080
  2. Immunogenetic and biological aspects of in vitro lymphocyte allotransformation (MLR) in the mouse.
    Transplant Rev. 1973;15:62-88 PMID: 4130133
  3. Interaction antigens detected by cytotoxic T cells with the major histocompatibility complex as modifier.
    Nature. 1975 Jul 31;256(5516):419-21 PMID: 1079924
  4. Quantitative analysis of the 51Cr release cytotoxicity assay for cytotoxic lymphocytes.
    Cell Immunol. 1974 Nov;14(2):284-302 PMID: 4219591
  5. In vitro cell-mediated immune responses to the male specific(H-Y) antigen in mice.
    J Exp Med. 1975 Nov 1;142(5):1108-20 PMID: 1081575
  6. Heterologous sera: a target for in vitro cell-mediated cytotoxicity.
    J Immunol. 1976 Jun;116(6):1561-5 PMID: 178800
  7. Secondary in vitro responses of T lymphocytes to non-H-2 alloantigens self-H-2-restricted responses induced in heterologous serum are not dependent on primary-stimulating non-H-2 alloantigens.
    J Exp Med. 1977 Apr 1;145(4):802-18 PMID: 300775
  8. Quantitative studies of the activation of cytotoxic lymphocyte precursor cells.
    Immunol Rev. 1977;35:38-58 PMID: 330390
  9. Primary in vitro cell-mediated lympholysis reaction of NZB mice against unmodified targets syngeneic at the major histocompatibility complex.
    J Exp Med. 1978 May 1;147(5):1435-48 PMID: 349111
  10. Mouse T cell-mediated cytolysis specifically triggered by cytophilic xenogeneic serum determinants: a caveat for the interpretation of experiments done under "syngeneic" conditions.
    J Immunol. 1978 Dec;121(6):2533-8 PMID: 82588
  11. T-T-cell interactions during the vitro cytotoxic allograft responses. I. Soluble products from activated Lyl+ T cells trigger autonomously antigen-primed Ly23+ T cells to cell proliferation and cytolytic activity.
    J Exp Med. 1978 Dec 1;148(6):1523-38 PMID: 309919
  12. Patterns of immune reactivity in autoimmune murine strains. I. Cell-mediated immune responses induced by H-2 indentical and H-2 incompatible stimulator cells.
    J Immunol. 1979 Jun;122(6):2319-27 PMID: 36433
  13. The in vitro generation and sustained culture of nude mouse cytolytic T-lymphocytes.
    J Exp Med. 1979 Jun 1;149(6):1460-76 PMID: 156241
  14. Primary in vitro cytotoxic response of NZB spleen cells to Qa-1b-associated antigenic determinants.
    J Exp Med. 1979 Dec 1;150(6):1555-60 PMID: 92525
  15. Xenoserum-induced cytolytic "T" cells: polyclonal specificity with an apparent "anti-self" component, and cooperative induction.
    Immunobiology. 1979 Aug;156(1-2):121-37 PMID: 317274
  16. Generation of primary cytotoxic lymphocytes against non-major histocompatibility complex antigens by anti-Ia serum plus complement-treated lymphocytes.
    J Exp Med. 1980 May 1;151(5):1305-10 PMID: 7373222
  17. Qed-1--a target for unrestricted killing by T cells.
    Eur J Immunol. 1980 Apr;10(4):289-98 PMID: 6967411
  18. On the T cell hyperreactivity of NZB mice against H-2-identical cells. Evidence for primary response characteristics and an increased helper potential.
    J Exp Med. 1980 Aug 1;152(2):296-305 PMID: 6967512
  19. Quantitation and cloning of cytolytic T lymphocytes and their precursors.
    Immunol Rev. 1980;51:93-123 PMID: 6448800
  20. Generation of cytotoxic lymphocytes to syngeneic tumor by using co-stimulator (Interleukin 2).
    J Immunol. 1980 Nov;125(5):1897-903 PMID: 6159395
  21. The use of a clonal assay for cytotoxic T lymphocytes to determine the Ly phenotypes of the cytotoxic precursor and effector cells to alloantigens and trinitrophenyl-modified self antigens.
    J Immunol. 1980 Nov;125(5):1977-86 PMID: 6159396
  22. Non-H-2 T-lymphocyte-mediated cytotoxicity after primary in vivo sensitization.
    Scand J Immunol. 1980;12(4):303-8 PMID: 6970411
  23. A simplified method for the production of murine T-cell growth factor free of lectin.
    J Immunol Methods. 1981;42(2):213-22 PMID: 6972416
  24. Lyt-23+ cyclophosphamide-sensitive T cells regulate the activity of an interleukin 2 inhibitor in vivo.
    J Exp Med. 1981 Aug 1;154(2):262-74 PMID: 6790656
  25. Recognition of polymorphic H-2 domains by T lymphocytes. I. Functional role of different H-2 domains for the generation of alloreactive cytotoxic T lymphocytes and determination of precursor frequencies.
    J Exp Med. 1981 Dec 1;154(6):1717-31 PMID: 6976406
  26. Enumeration of IL 2-secreting helper T cells by limiting dilution analysis, and demonstration of unexpectedly high levels of IL 2 production per responding cell.
    J Immunol. 1982 May;128(5):2258-64 PMID: 6977590
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.156.2.610
Published
1982-08-01
Pages
610-21
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186758
Subset
IM
Grants
NIA NIH HHS · AG-02152 · United States
NCI NIH HHS · CA-17818 · United States
NCI NIH HHS · CA-29803 · United States
External Links
PubMed source DOI: 10.1084/jem.156.2.610 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2